The Hairy Cells (HC) from four patients with Hairy Cell Leukemia (HCL) were studied. These cells exhibited membrane properties of B-lymphocytes; quantitation of surface membrane immunoglobulins by the peroxidase labeled antibody technique showed an average number of 20,000 to 40,000 immunoglobulin molecules per marked cell. Observations by scanning electron microscopy (SEM) showed that HC have a great capacity to adhere quickly to glass coverslips, to phagocytose latex particles, and to resist trypsin treatment. These cells possess numerous inter cellular connections forming a network on the glass-coverslip, and thin cytoplasmic projections enabling them to adhere. When cells adhering to a support are stimulated by latex particles, they are able to phagocytose these particles and this results in the swelling of the cell due to the unpleating of numerous folds of the membrane. After this stimulation, HC appeared smooth. Our SEM analysis provides an explanation of the disappearance of the hairy appearance classically described. After trypsin treatment, the adhering HC remain on the glass even if they have suffered membrane damage. In conclusion, SEM examination of adhering cells and phagocytosis demonstrate the variability of the membrane aspect of the HCL cells under various culture conditions.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Bioinspired complex cellulose nanorod-architectures: A model for dual-responsive smart carriers.
Carbohydr Polym
March 2025
Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montreal, QC H3A 0B8, Canada; Quebec Centre for Advanced Materials (QCAM) and Pulp and Paper Research Centre, McGill University, 3420 University Street, Montreal, QC H3A 2A7, Canada. Electronic address:
The synergy between nanomaterials as solid supports and supramolecular concepts has resulted in nanomaterials with hierarchical structure and enhanced functionality. Herein, we developed and investigated innovative supramolecular functionalities arising from the synergy between organic moieties and the preexisting nanoscale soft material backbones. Based on these complex molecular nano-architectures, a new nanorod carbohydrate polymer carrier was designed with bifunctional hairy nanocellulose (BHNC) to reveal dual-responsive advanced drug delivery (ADD).
View Article and Find Full Text PDF[Mechanism of ginsenoside Rg_1 in regulating autophagy through miR-155/Notch1/Hes1 pathway to attenuate hypoxia/reoxygenation injury in HL-1 cells].
Zhongguo Zhong Yao Za Zhi
December 2024
School of Traditional Chinese Medicine, Binzhou Medical College Yantai 264003, China Institute of Basic Medicine, Xiyuan Hospital, China Academy of Chinese Medical Sciences Beijing 100091, China.
This article explored the specific mechanism by which ginsenoside Rg_1 regulates cellular autophagy to attenuate hypoxia/reoxygenation(H/R) injury in HL-1 cardiomyocytes through the microRNA155(miR-155)/neurogenic gene Notch homologous protein 1(Notch1)/hairy and enhancer of split 1(Hes1) pathway. An HL-1 cell model with H/R injury was constructed, and ginsenoside Rg_1 and/or Notch1 inhibitor DAPT and miR-155 mimics were used to treat cells. Cell counting kit(CCK)-8 was used to detect the relative viability of HL-1 cells with H/R injury.
View Article and Find Full Text PDFExploring the Tomato Root Protein Network Exploited by Core Type 3 Effectors from the Species Complex.
J Proteome Res
January 2025
Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.
Proteomics has become a powerful approach for the identification and characterization of type III effectors (T3Es). Members of the species complex (RSSC) deploy T3Es to manipulate host cells and to promote root infection of, among others, a wide range of solanaceous plants such as tomato, potato, and tobacco. Here, we used TurboID-mediated proximity labeling (PL) in tomato hairy root cultures to explore the proxeomes of the core RSSC T3Es RipU, RipD, and RipB.
View Article and Find Full Text PDFIdentification of Quantitative Trait Loci (QTLs) and candidate genes for trichome development in Brassica villosa using genetic, genomic, and transcriptomic approaches.
Mol Genet Genomics
January 2025
Department of Molecular Phytopathology and Biotechnology, Institute of Phytopathology, Christian-Albrechts-University of Kiel, 24118, Kiel, Germany.
Brassica villosa is characterized by its dense hairiness and high resistance against the fungal pathogen Sclerotinia sclerotiorum. Information on the genetic and molecular mechanisms governing trichome development in B. villosa is rare.
View Article and Find Full Text PDFPuerarin pretreatment provides protection against myocardial ischemia/reperfusion injury via inhibiting excessive autophagy and apoptosis by modulation of HES1.
Sci Rep
January 2025
Department of Cardiovascular Surgery, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, 17 Yongwai Road, Nanchang, 330006, Jiangxi, China.
The study aimed to elucidate the underlying pharmacological mechanism of the traditional Chinese medicine Pue in ameliorating myocardial ischemia-reperfusion injury (MIRI), a critical clinical challenge exacerbated by reperfusion therapy. In vivo MIRI and in vitro anoxia/reoxygenation (A/R) models were constructed. The results demonstrated that Pue pretreatment effectively alleviated MIRI, as manifested by diminishing the levels of serum CK-MB and LDH, mitigating the extent of myocardial infarction and enhancing cardiac functionality.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!