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BIF-induced ROS-mediated cytotoxicity and genotoxicity in embryonic cell culture of Daphnia magna.
Aquat Toxicol
March 2025
National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Cochin-16, Kerala, India. Electronic address:
Bifenthrin (BIF) is a widely used synthetic pyrethroid insecticide that poses significant risks to the environment, particularly to aquatic ecosystems. In the present study, the cytotoxic and genotoxic effects of BIF on Daphnia magna cells were evaluated using in vitro methods. To achieve this, we developed a novel embryonic cell culture system from D.
View Article and Find Full Text PDFEffects of Globularia cordifolia on colon cancer cells: antioxidant activity, inflammatory cytokine analysis and molecular docking studies.
J Sci Food Agric
December 2024
Faculty of Veterinary Medicine Department of Biochemistry, Ondokuz Mayis University, Samsun, Turkey.
Background: The present study aimed to comprehensively evaluate the anticancer, anti-inflammatory, and antioxidant properties of Globularia cordifolia L.
Samples: The plant material was collected and extracted using the maceration method. Antioxidant activities were assessed through DPPH (i.
Blockade of store-operated calcium entry by BTP2 preserves anti-inflammatory gene expression in human peripheral blood mononuclear cells.
Hum Immunol
November 2024
Division of Nephrology and Hypertension, Department of Medicine, NewYork-Presbyterian-Weill Cornell Medicine, New York, NY, USA; Department of Transplantation Medicine, NewYork-Presbyterian Hospital-Weill Cornell Medicine, New York, NY, USA. Electronic address:
Store-operated calcium entry (SOCE) is essential for cellular signaling. Earlier studies of the pyrazole derivative BTP2, an efficient inhibitor SOCE, identified that SOCE blockade suppresses proinflammatory gene expression. The impact of SOCE blockade on gene expression at the whole transcriptome level, however, is unknown.
View Article and Find Full Text PDFInhibition of Development and Metabolism of Dual-Species Biofilms of and () by Organoselenium Compounds.
Pharmaceuticals (Basel)
August 2024
Instituto de Pesquisa & Desenvolvimento-IP&D, Universidade do Vale do Paraíba-UNIVAP, Av. Shishima Hifumi, 2911, São José dos Campos 12244-390, SP, Brazil.
Although is the most frequently identified species in clinical settings, a significant number of infections related to the non- (NAC) species, , has been reported. Both species are able to produce biofilms and have been an important resistance-related factor to antimicrobial resistance. In addition, the microbial relationship is common in the human body, contributing to the formation of polymicrobial biofilms.
View Article and Find Full Text PDFXTT assay for detection of bacterial metabolic activity in water-based polyester polyurethane.
PLoS One
June 2024
Departamento de Microbiología Molecular, Instituto de Biotecnología, Cuernavaca, Morelos, México.
Cellular metabolic activity can be detected by tetrazolium-based colorimetric assays, which rely on dehydrogenase enzymes from living cells to reduce tetrazolium compounds into colored formazan products. Although these methods have been used in different fields of microbiology, their application to the detection of bacteria with plastic-degrading activity has not been well documented. Here, we report a microplate-adapted method for the detection of bacteria metabolically active on the commercial polyester polyurethane (PU) Impranil®DLN using the tetrazolium salt 2,3-bis [2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT).
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