A study was made of the chromatin structure of mature sperm cells from healthy males aged 25 to 40 using fluorescent microscopy and acridine orange staining according to the DNP cell thermal denaturation technique modified by the authors. It was shown that normal human sperm cell chromatin melting profiles represent uniform curves with maxima in the following temperature ranges: 43 (+/- 2) degrees, 55 (+/- 1) degrees, 67 (+/- 2) degrees, 77 (+/- 1) degrees, 82 (+/- 0.5) degrees, 89 (+/- 1) degrees, 92 (+/- 2) degrees (P less than 0.01), that are identical to those obtained with lymphocytes of healthy males with certain deviations from the standard normal variant. No heteromorphism was revealed in the sperm cell chromatin. Marked polymorphism in the chromatin structure occurs but at the diploid cell level. A 10-time decrease in the fluorescence of AO bound with sperm cell chromatin as compared to F530 AO bound with lymphocyte chromatin structure of the same individual supports the data on the over condensation of sperm cell nuclear chromatin as compared to that in lymphocytes.
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