Eight nonspecific agglutinating enterobacterial strains have been classified with the definite taxon on the basis of the data obtained by the study of their biochemical and serological properties. The possibilities offered by the supplementary methods for the study of such strains in practical laboratories are discussed.
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Heliyon
January 2025
Department of Otorhinolaryngology Head and Neck Surgery, Children's Hospital Capital Institute of Pediatrics, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
(, Hi) is an opportunistic bacterium that colonizes the upper respiratory tract of humans and frequently causes meningitis, pneumonia, sepsis, and other severe infections in children. Early and accurate detection of is essential for effective diagnosis and treatment. In this study, we established a novel diagnostic method by integrating the CRISPR-Cas12a detection platform with multiple cross-displacement amplification (MCDA), termed the Hi-MCDA-CRISPR assay.
View Article and Find Full Text PDFData Brief
February 2025
Department of Child Health, Faculty of Medicine, Dr. Cipto Mangunkusumo Hospital, University of Indonesia, Jakarta 10430, Indonesia.
Glycogen storage disease type IV (GSD IV) is a rare disease caused by a defect in glycogen branching enzyme 1 (GBE1), which played a crucial role in glycogen branching. GSD IV occurs once in approximately 1 in every 760,000 to 960,000 live births and is inherited in an autosomal recessive pattern. Early diagnosis of GSD IV is challenging due to non-specific symptoms, such as liver and spleen enlargement, which can overlap with other hematologic and hepatobiliary disorders.
View Article and Find Full Text PDFFront Immunol
January 2025
Department of Neurology, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Background And Objective: Neurobrucellosis is a rare neurological disorder characterized by diverse clinical manifestations. Although several relevant cases were reported, our understanding of this disorder is limited. In this study, we presented the clinical and imaging characteristics of four cases of neurobrucellosis.
View Article and Find Full Text PDFCureus
December 2024
Anesthesiology, Intensive Care and Emergency Department, Centro Hospitalar Universitário de Santo António, Porto, PRT.
Acute pulmonary embolism (PE) is a significant cause of cardiac arrests, with subsequent high mortality worldwide. Early recognition of acute PE allows earlier diagnosis, stabilization, and risk stratification, which are crucial in deciding the most adequate treatment option. However, diagnosis is sometimes difficult due to nonspecific clinical presentation.
View Article and Find Full Text PDFAnalyst
January 2025
Institutes of Biomedical Sciences & Shanghai Stomatological Hospital, Department of Chemistry, Fudan University, Shanghai 200433, China.
Reducing the time required for the detection of bacteria in blood samples is a critical area of investigation in the field of clinical diagnosis. Positive blood culture samples often require a plate culture stage due to the interference of blood cells and proteins, which can result in significant delays before the isolation of single colonies suitable for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis. In this study, we developed a non-specific enrichment strategy based on SiO-encapsulated FeO nanoparticles combined with MALDI-TOF MS for direct identification of bacteria from aqueous environments or positive blood culture samples.
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