A cholinesterase activity was revealed in two to four regions of each of the 120 fibers isolated from the frog sartorius muscle, which suggests polysynaptic character of innervation of the phasic muscle fibers. Electrophysiological methods showed the participation of different axons in formation of synapses with a single fiber, as well as the existence of synapses with the correlation between EPP's amplitude and strength of stimulation. The data obtained show such EPPs to be connected with polyaxonal innervation of one region of a muscle fiber.
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Biomed Phys Eng Express
January 2025
Children's Hospital of Eastern Switzerland, Claudiusstrasse 6, St.Gallen, 9006, SWITZERLAND.
Mapping the myomagnetic field of a straight and easily accessible muscle after electrical stimulation using triaxial optically pumped magnetometers (OPMs) to assess potential benefits for magnetomyography (MMG). Approach: Six triaxial OPMs were arranged in two rows with three sensors each along the abductor digiti minimi (ADM) muscle. The upper row of sensors was inclined by 45° with respect to the lower row and all sensors were aligned closely to the skin surface without direct contact.
View Article and Find Full Text PDFAm J Physiol Cell Physiol
January 2025
Smooth Muscle Research Centre, Dundalk Institute of Technology, Dundalk, Ireland.
Adrenergic stimulation induces contractions in the corpus cavernosum smooth muscle (CCSM) that are important in maintaining penile flaccidity. The aim of this study was to investigate the role of K7 channels in regulating contractions and their underlying Ca signals in mouse CCSM. Quantitative PCR revealed transcriptional expression of KCNQ1 and KCNQ3-5 genes in whole CCSM, with KCNQ5 as the most highly transcribed K7 encoding gene.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
January 2025
Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, CA 94720.
Norepinephrine in vertebrates and its invertebrate analog, octopamine, regulate the activity of neural circuits. We find that, when hungry, larvae switch activity in type II octopaminergic motor neurons (MNs) to high-frequency bursts, which coincide with locomotion-driving bursts in type I glutamatergic MNs that converge on the same muscles. Optical quantal analysis across hundreds of synapses simultaneously reveals that octopamine potentiates glutamate release by tonic type Ib MNs, but not phasic type Is MNs, and occurs via the G-coupled octopamine receptor (OAMB).
View Article and Find Full Text PDFInt Neurourol J
December 2024
Experimental Physiology Laboratory, Superior Institute of Biomedical Sciences, State University of Ceará, Fortaleza, Brazil.
Purpose: This investigation was conducted to elucidate the effects of eugenol on bladder contractility through experimental and in silico approaches.
Methods: To assess the impact of eugenol on muscular contractility, longitudinal strips of bladder tissue, measuring 2 mm by 6 mm, were mounted in perfusion chambers connected to an isometric force transducer. Furthermore, molecular docking studies were conducted to explore the potential of eugenol to target the M3 muscarinic acetylcholine receptor (M3R) and voltage-operated calcium channels (VOCCs) in muscle cells, utilizing in silico techniques.
Dev Cell
December 2024
Edwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA. Electronic address:
Lymphatic muscle cells (LMCs) within the wall of collecting lymphatic vessels exhibit tonic and autonomous phasic contractions, which drive active lymph transport to maintain tissue-fluid homeostasis and support immune surveillance. Damage to LMCs disrupts lymphatic function and is related to various diseases. Despite their importance, knowledge of the gene transcriptional signatures in LMCs and how they relate to lymphatic function in normal and disease contexts is largely missing.
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