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Bioanalytical method for NAD detection in blood plasma utilizing solution-phase formate dehydrogenase and electrochemical detection.
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January 2025
School of Chemistry, Institute of Science, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand.
Nicotinamide adenine dinucleotide is a crucial coenzyme in cellular metabolism and is implicated in various diseases. This work introduces an electrochemical bioanalytical method utilizing solution-phase formate dehydrogenase (CbFDH) for detecting its oxidized form (NAD) in human blood plasma samples. The detection mechanism involves the catalytic conversion of NAD to NADH, facilitated by CbFDH in the presence of formate.
View Article and Find Full Text PDFNDUFA11 may be the disulfidptosis-related biomarker of ischemic stroke based on integrated bioinformatics, clinical samples, and experimental analyses.
Front Neurosci
January 2025
Department of Geriatric Rehabilitation, Jiangbin Hospital, Nanning, China.
Background: Programmed cell death plays an important role in neuronal injury and death after ischemic stroke (IS), leading to cellular glucose deficiency. Glucose deficiency can cause abnormal accumulation of cytotoxic disulfides, resulting in disulfidptosis. Ferroptosis, apoptosis, necroptosis, and autophagy inhibitors cannot inhibit this novel programmed cell death mechanism.
View Article and Find Full Text PDFFirst Report of Apple Chlorotic Leaf Spot Virus in Pome and Stone Fruit Trees in Azerbaijan.
Plant Dis
January 2025
Cornell University, Ithaca, New York, United States;
Azerbaijan is major producer of fruit crops, such as pome and stone fruits, in the Caspian Sea and Caucasus Mountains areas (FAO Stat, 2022). No information is available on the occurrence of apple chlorotic leaf spot virus (ACLSV, genus Trichovirus, family Betaflexiviridae) in the country. Therefore, the main fruit tree production areas in Azerbaijan were surveyed for ACLSV during the 2017-2019 growing seasons by DAS-ELISA using ACLSV reagents (Neogen - Scotland, UK) (Clark and Adams 1977).
View Article and Find Full Text PDFCharacterization of the oxygen-tolerant formate dehydrogenase from .
Front Microbiol
January 2025
Department of Plant Physiology, Institute of Biosciences, University of Rostock, Rostock, Germany.
Fixation of CO into the organic compound formate by formate dehydrogenases (FDHs) is regarded as the oldest autotrophic process on Earth. It has been proposed that an FDH-dependent CO fixation module could support CO assimilation even in photoautotrophic organisms. In the present study, we characterized FDH from (FDH) due to its ability to reduce CO under aerobic conditions.
View Article and Find Full Text PDFBackground: Atherosclerosis (AS) is caused by the endothelium injury associated with oxidative stress. Previous studies have shown that the Phlegm-Eliminating and Stasis- Transforming Decoction (Huayu Qutan Decoction, HYQTD) has mitochondrial protective function. The objective of this research was to explore how HYQTD drug-containing serum (HYQTD-DS) could potentially protect mitochondrial energy production in endothelial cells (ECs) from injury caused by hydrogen peroxide (H2O2)-induced oxidative damage in AS through SIRT1/PGC-1α/ Nrf2 pathway.
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