Interaction between mutagenic effects of strong homogeneous magnetic fields and fission neutron-plus-gamma radiation was investigated, using the sex-linked, recessive, lethal test in Drosophila melanogaster. Drosophila males were exposed chronically, for seven days, to a 37,000-G homogeneous magnetic field and/or 3 doses of neutron-plus gamma radiation. Mutations in spermatozoa, spermatids, and spermatocytes were scored. There was no evidence of interaction between the effects of the two types of exposure in causing genetic damage in any of the three cell types. Dose-response relations for the radiation doses were linear for spermatozoa and spermatids for all three doses and for spermatocytes, up to 300 rads. Spermatozoa appear to be most sensitive to neutron-radiation-induced, recessive lethal mutations.
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http://dx.doi.org/10.1002/em.2860020210 | DOI Listing |
J Pharm Pharmacol
January 2025
Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Objectives: PD15, a novel natural steroidal saponin extracted from the rhizomes of Paris delavayi Franchet, has demonstrated a strong cytotoxic effect against HepG2 and U87MG cells. However, its therapeutic effects on colorectal cancer (CRC) and the underlying molecular mechanisms remain unclear.
Methods: MTT assay, clonogenic assay, Hoechst 33258 staining, flow cytometry, molecular docking, and western blot were used to investigate the mechanism of PD15 in HCT116 cell lines.
Database (Oxford)
January 2025
Research and Development Centre, Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON CA K1A 0C6, Canada.
It is well-known that the use of vocabulary in phenotype treatments is often inconsistent. An earlier survey of biologists who create or use phenotypic characters revealed that this lack of standardization leads to ambiguities, frustrating both the consumers and producers of phenotypic data. Such ambiguities are challenging for biologists, and more so for Artificial Intelligence, to resolve.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
February 2025
Department of Earth Sciences, University College London, London WC1E 6BT, United Kingdom.
CaSiO[Formula: see text] perovskite (CaPv) is the last major mineral in the Earth's lower mantle whose elasticity remains largely unresolved. Here, we investigate the elasticity of CaPv using ab initio machine-learning force fields (MLFF). At room temperature, the elasticity of tetragonal CaPv determined by MLFF molecular dynamics (MD) agrees well with experimental measurements after considering temperature induced variations in the hydrostatic structure, proving the effectiveness of the method.
View Article and Find Full Text PDFFree Radic Res
January 2025
Department of Human and Animal Physiology, Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia.
Reactive oxygen species (ROS) produced by NADPH oxidase promote contraction of peripheral arteries, which is especially pronounced in early postnatal period in comparison to adulthood, but the mechanisms of such vasomotor influence are poorly understood. We tested the hypothesis that Rho-kinase and protein kinase C (PKC) mediate procontractile influence of NADPH oxidase derived ROS in peripheral artery of early postnatal rats. In addition, we evaluated the involvement Src-kinase and L-type voltage-gated Ca channels (LTCC) into procontractile influence of ROS, produced by NADPH oxidase, because of their known interplay with Rho-kinase and PKC pathways.
View Article and Find Full Text PDFMol Pharm
January 2025
Regional Center of Advanced Technologies and Materials, Czech Advanced Technology and Research Institute (CATRIN), Palacký University Olomouc, Šlechtitelů 27, 779 00 Olomouc, Czech Republic.
Lipid-mediated delivery of active pharmaceutical ingredients (API) opened new possibilities in advanced therapies. By encapsulating an API into a lipid nanocarrier (LNC), one can safely deliver APIs not soluble in water, those with otherwise strong adverse effects, or very fragile ones such as nucleic acids. However, for the rational design of LNCs, a detailed understanding of the composition-structure-function relationships is missing.
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