A new procedure is presented for the isolation and purification of A-type staphylococcus enterotoxin. Homogeneous enterotoxin preparation was obtained by purification in 2 phases. In radial double agar-gel immunodiffusion the smallest precipitating dose of the isolated and purified enterotoxin was found to be 1.4-0.7 micrograms protein and 0.4-0.1 micrograms nitrogen. In cat experiments the dose giving a positive reaction was 2 micrograms protein or 0.5 micrograms nitrogen calculated for kg body weight.
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