P. aeruginosa flagella of the complex serotype a (a0, a2, a3) and the uniform serotype b were isolated, purified, and depolymerized by heat. The flagellins obtained form one precipitin band each and show reactions of non-identity in Ouchterlony tests. The immunologic reaction is not altered by treatment with SDS and mercaptoethanol. In disc microelectrophoresis and SDS gradient gel microelectrophoresis on polyacrylamide gels, the flagellins migrate as homogeneous proteins. They have identical electrophoretic mobilities and a molecular weight of about 50,000.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/BF02122856 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
How Technical Advances Changed the Concept of Antibodies.
Immunol Rev
November 2024
Institute of Philosophy of the Czech Academy of Sciences, Prague, Czech Republic.
Shaped by advances in scientific instrumentation and experimental techniques, the concept of antibody has undergone profound transformations throughout the history of immunology. Serological assays, separation techniques, protein fragmentation techniques, molecular biology techniques, and other methodological innovations did not only serve to produce data on the structure and function of these molecules but, by framing antibodies into a unique facet of experimental investigation, were effectively redefining and reconceptualizing these molecules for the scientific community. The characteristics and properties of antibodies observed in experimental settings were often directly extrapolated to their presumed nature in living organisms, as exemplified by the literal identification of antibodies with a gamma electrophoretic fraction in the 1930s.
View Article and Find Full Text PDFSerum protein electrophoretic profile changes in West Nile virus-naturally infected horses.
J Equine Vet Sci
January 2025
Department of Clinical Sciences, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 11 Stavrou Voutyra str., Thessaloniki, Greece, 546 27.
Serum protein electrophoresis (SPE) is widely used to evaluate protein changes associated with several pathologies, aiming to assist clinical diagnosis. This study determines, for the first time, SPE profile changes in West Nile virus-naturally infected horses. Sixty horses with different clinical and infection status (encephalitis, asymptomatic infection, and immunologically naïve) were included.
View Article and Find Full Text PDFHepatocellular carcinoma antibodies preferably identify nitro-oxidative-DNA lesions induced by 4-Chloro-orthophenylenediamine and DEANO.
Sci Rep
November 2024
Department of Biochemistry, Faculty of Medicine, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, Uttar Pradesh, 202002, India.
The widespread use of oxidative hair colouring cosmetics threatens public health. Phenylenediamine derivatives serve as the main pigment in permanent hair colours. They interact with biological macromolecules, altering their functional and structural physiology.
View Article and Find Full Text PDFGluten Is Not Gluten.
Nutrients
August 2024
Department of Bioactive and Functional Food Chemistry, Institute of Applied Biosciences, Karlsruhe Institute of Technology (KIT), 76131 Karlsruhe, Germany.
Wheat gluten is responsible for the unique baking properties of wheat flour, but it also causes wheat-related disorders in predisposed individuals. Different commercially available gluten materials are commonly used for a variety of assays, but a detailed characterization of their composition is missing in many cases. This is why we aimed to provide an in-depth analysis of three commonly used gliadin and gluten materials from two different batches using gel electrophoretic and chromatographic techniques.
View Article and Find Full Text PDFRhesus Macaques: VNTR Polymorphism of the FCGRT Gene.
Bull Exp Biol Med
July 2024
Kurchatov Complex of Medical Primatology, National Research Center "Kurchatov Institute", Sochi, Russia.
Variable number tandem repeat (VNTR) polymorphisms of the human neonatal IgG Fc receptor α-chain gene (FCGRT) are known to influence the expression levels of FCGRT and IgG in serum. Monkeys are considered to be a relevant biological model for studying the effects of immunobiological drugs. The study determined the functional VNTR polymorphisms of the FCGRT gene in 109 male rhesus macaques from the nursery of the Kurchatov Complex of Medical Primatology.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!