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Bio-nanopore technology for biomolecules detection.
Adv Biotechnol (Singap)
December 2024
School of Food Science and Technology, State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, 214122, Jiangsu, China.
Bio-nanopore technology holds great promise in biomacromolecule detection, with its high throughput and low cost positioning it as an ideal detection tool. This technology employs a unique detection mechanism that utilizes nanoscale pores to rapidly and sensitively convert biological molecules interactions into electrical signals, enabling real-time, single-molecule detection with exceptional sensitivity. This review focuses on the latest advancements in this technology across various domains, including DNA and RNA sequencing, protein detection, and small molecule identification.
View Article and Find Full Text PDFIntermittent Fetal Heart Monitoring Through Moyo Doppler Improves Nonreassuring Fetal Heart Rate Detection in Hospital of Ethiopia: A Randomized Controlled Trial.
J Perinat Neonatal Nurs
January 2025
Author Affiliations: Departments of Midwifery (Mr Fikadu), Internal Medicine (Dr Shibru), and Obstetrics and Gynecology (Drs Koira, Chufamo, and Mulugeta), and School of Public Health (Messrs Kote and Hailemariam and Dr Ayele), College of Medicine and Health Sciences, Arba Minch University, Southern Region, Arba Minch, Ethiopia; Ethiopia Midwives Association, Addis Ababa, Ethiopia (Messrs Belgu and Mazengia); and Institute of Public Health, College of Medicine and Health Sciences, University of Gondar, Amhara Region, Gondar, Ethiopia (Dr Ayele).
Purpose: This study was aimed to assess the effect of intermittent fetal heart rate (FHR) monitoring using Moyo Doppler compared with fetoscope in hospitals of Ethiopia, 2023.
Background: To facilitate more prompt identification of a hypoxic fetus, Laerdal Global Health has recently introduced the Moyo FHR monitor. Nevertheless, there exists limited knowledge regarding its efficacy derived from multicenter contextual trials conducted in resource-constrained environments, specifically in Ethiopia.
Developing a novel TaqMan qPCR assay for optimizing Pullorum detection in chickens.
Vet Q
December 2025
Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, China.
Pullorum, the causative agent of pullorum disease, posing a significant threat to the global production of poultry meat and eggs. However, existing detection methods have substantial limitations in efficiency and accuracy. Herein, we developed a genomic deletion-targeted TaqMan qPCR assay for identification of Pullorum, enabling precise differentiation from other serovars.
View Article and Find Full Text PDFConcurrent presence of Staphylococcal Cassette Chromosome types of Meticillin-Resistant in hospital environments and post-operative patients at a hospital in Kathmandu, Nepal.
Infect Prev Pract
March 2025
Central Department of Biotechnology, Tribhuvan University, Kirtipur, Nepal.
Introduction: Meticillin resistant (MRSA) is a major contributor to surgical site infections in post-operative patients. Hospital environments harbor MRSA, contributing to higher risk of nosocomial infections. Meticillin resistance is conferred by acquisition of gene, typically carried on mobile genetic element called Staphylococcal Cassette Chromosome (SCC).
View Article and Find Full Text PDFSuppression of MCP-1, IFN-γ and IL-6 production of HNSCC by pembrolizumab added to docetaxel and cisplatin (TP) exceeding those of TP alone is linked to improved survival.
Front Immunol
January 2025
Department of Otorhinolaryngology, Head and Neck surgery, University Hospital Leipzig, Leipzig, Germany.
Background: Adding pembrolizumab, an anti-PD-1 antibody approved for treatment of head and neck squamous cell carcinoma (HNSCC) to neoadjuvant (induction-) chemotherapy utilizing docetaxel and cisplatin (TP) followed by radiotherapy may improve outcome in larynx organ-preservation (LOP) that is investigated in the European Larynx-Organ preservation Study (ELOS). As biomarkers for response to TP and pembrolizumab +TP are missing but may include cytokines, this work aims on determining cytokines potentially linked to outcome as prognostic markers sufficient to predict and/or monitor response to successful LOP.
Methods: Collagenase IV digests were generated from 47 histopathological confirmed HNSCC tumor samples and seeded in 96-well plates containing pembrolizumab, docetaxel, cisplatin either solely or in binary or ternary combination.
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