AI Article Synopsis
- A variant of Streptomyces griseus, strain 45-H, produces a compound called factor C that can stimulate conidium formation in a mutant strain that normally does not produce it.
- Factor C can be quantitatively measured based on its ability to induce this conidium formation.
- The purification process involves ion-exchange and affinity chromatography, resulting in a concentrated substance with a molecular weight of approximately 34,500 and a hydrophobic amino acid composition of 60%.
Article Abstract
A conidium-producing variant of Streptomyces griseus, strain 45-H, produces a substance, factor C, which is capable of inducing conidium formation in the hyphae of a conidium-non-producing mutant, strain 52-1. Factor C can be determined quantitatively on the basis of this biological effect. The biologically active substance can be purified by ion-exchange chromatography on cellulose phosphate combined with affinity chromatography on DNA-agarose. The purified substance is concentrated at least 1700 times. The molecular weight of factor C, estimated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate, is about 34500. On determining the amino acid composition of factor C 60% of the amino acids were found to be hydrophobic.
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| http://dx.doi.org/10.1111/j.1432-1033.1980.tb04322.x | DOI Listing |
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