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Development and Application of a Fully Automated Chemiluminescence Enzyme Immunoassay for the Detection of Antibodies Against Porcine Circovirus 3 Cap.
Viruses
December 2024
State Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Center for Swine Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Porcine circovirus 3 (PCV3) is a small non-enveloped circovirus associated with porcine dermatitis and nephropathy syndrome (PDNS). It has occurred worldwide and poses a serious threat to the pig industry. However, there is no commercially available vaccine.
View Article and Find Full Text PDFEvaluation of a commercial Histoplasma antigen detection enzyme immunoassay for the follow-up of histoplasmosis treatment in people living with HIV from Argentina.
Med Mycol
December 2024
Unidad Micología, Hospital de Enfermedades Infecciosas Dr. Francisco J. Muñiz, CABA, Argentina.
Article Synopsis
- Histoplasmosis is particularly dangerous for HIV patients, especially where antiretroviral therapy is hard to get, making antigen detection assays vital for quick diagnoses and reducing mortality.
- The study assessed 27 HIV patients with histoplasmosis over 24 weeks, monitoring urinary Histoplasma antigen levels as they received treatment with amphotericin B followed by itraconazole.
- Results indicated that as antigen levels decreased, patient clinical outcomes improved, suggesting that the clarus Histoplasma GM EIA kit could effectively monitor treatment responses, despite some patients having atypical antigen patterns due to immune system responses or medication absorption issues.
Comparison of the STANDARD M10 . , Xpert . , and BD MAX Cdiff assays as confirmatory tests in a two-step algorithm for diagnosing infection.
Microbiol Spectr
January 2025
Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea.
Current guidelines recommend a two-step algorithm rather than relying solely on a single test for diagnosing infection. This algorithm starts with enzyme immunoassay (EIA) for detecting glutamate dehydrogenase (GDH) and toxins A/B, followed by nucleic acid amplification test (NAAT) for GDH-positive but toxin-negative cases. This study compared the performance of three commercial NAATs: the STANDARD M10 , Xpert , and BD MAX Cdiff assays, utilized as confirmatory testing of the two-step algorithm.
View Article and Find Full Text PDFDevelopment and validation of a VP7-specific EIA for determining the potency and stability of inactivated rotavirus vaccine.
J Virol Methods
February 2025
Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address:
Article Synopsis
- Researchers developed a new enzyme immunoassay (EIA) to evaluate the potency of inactivated rotavirus vaccines using a specific monoclonal antibody that targets RV VP7, a crucial protein for vaccine efficacy.
- The EIA demonstrated excellent specificity and accuracy, with a low detection limit, making it reliable for assessing the stability and potency of the vaccine under different storage conditions.
- This assay could replace animal testing for potency checks, providing a feasible quality control method for clinical trials involving inactivated rotavirus vaccine lots.
Rapid and specific screening of monoclonal antibodies in hybridoma supernatants by an enzyme-based dipstick immunoassay.
Talanta
March 2025
Department of Biotechnology Engineering, Faculty of Engineering Science, Ben-Gurion University of the Negev, Beer-Sheva, 8410501, Israel. Electronic address:
Hybridoma technology remains a cornerstone of monoclonal antibody (mAb) discovery. Classical screening practices such as ELISA, western blot, and dot blot require laborious, time-consuming procedures, rendering them inefficient in time-restricted decision-making. Additionally, due to these assays' practical and technical limitations, specificity testing of the mAbs is usually omitted during the primary screening of hybridoma libraries.
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