There are several occasions when it may be useful to preserve skin grafts for at least 15 days, in a good enough state to provide viable autografts. We describe a technique of cryopreservation which facilitates this type of procedure. Stretched sheets of skin are incubated in a protective medium. Freezing is programmed with cooling rates of 1 degree/min first to 50 degrees C and 5 degrees/min thereafter. The skin is placed in bags that are stored in liquid nitrogen and when required for use is rapidly thawed. Thin and ultra-thin sections of this preserved skin were studied by light and electron microscopy. Cultures wer made from thawed skin and growing cells were found to contain epithelial-like or fibroblast-like elements. When pieces of thawed skin were replaced on their original donor site the autograft was successful. The technique we have described gave consistently good results and its clinical applications need further development and study.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0007-1226(82)90058-3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
From Cartilage to Matrix: Protocols for the Decellularization of Porcine Auricular Cartilage.
Bioengineering (Basel)
January 2025
Postgraduate Program in Structural and Functional Interactions in Rehabilitation, Postgraduate Department, University of Marilia (UNIMAR), Marilia 17525-902, Brazil.
The shortage of tissues and damaged organs led to the development of tissue engineering. Biological scaffolds, created from the extracellular matrix (ECM) of organs and tissues, have emerged as a promising solution for transplants. The ECM of decellularized auricular cartilage is a potential tool for producing ideal scaffolds for the recellularization and implantation of new tissue in damaged areas.
View Article and Find Full Text PDFEnhanced post-thaw functional characteristics of cryopreserved caprine dermal fibroblasts through adhesion to extracellular matrix proteins.
Mol Biol Rep
January 2025
Goat Genetics and Breeding Division, ICAR-Central Institute for Research On Goats, Makhdoom, Farah, Mathura, 281 122, Uttar Pradesh, India.
Background: Extracellular matrix (ECM) proteins play a crucial role in regulating the biological properties of adherent cells. For cryopreserved fibroblasts, a favourable ECM environment can help restore their natural morphology and function more rapidly, minimizing post-thaw stress responses.
Methods And Results: This study explored the functional responses of cryopreserved enriched caprine adult dermal fibroblast (cadFibroblast) cells to structural [collagen-IV and rat tail collagen (RTC)] and adhesion ECM proteins (laminin, fibronectin, and vitronectin) under in vitro culture conditions.
Fluorescence of various buried fresh and fresh-frozen-thawed tissue types up until the point of active decay: a human taphonomy study.
Int J Legal Med
December 2024
Department of Medical Biology, Section Clinical Anatomy and Embryology, AmsterdamUMC, Location Academic Medical Centre, Meibergdreef 9, Amsterdam, AZ, 1105, The Netherlands.
Forensic taphonomy is the study of postmortem changes of human remains for the purpose of answering legal investigative questions. Many variables can affect the pattern and rate of decomposition of remains, posing challenges for taphonomic studies and estimation of the postmortem interval. Given the gap in knowledge regarding the suitability of using frozen remains to extrapolate conclusions to fresh material, investigating the effects of freeze-thaw cycles followed by burial on human remains is vital for forensic practice and taphonomic research.
View Article and Find Full Text PDFCryopreserved PBMCs can be used for the analysis of mitochondrial respiration and serve as a diagnostic tool for mitochondrial diseases.
Anal Biochem
March 2025
Laboratory of Bioenergetics, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic. Electronic address:
Article Synopsis
- Mitochondrial diseases are serious inherited disorders primarily affecting children, linked to issues with the mitochondrial energy production system known as oxidative phosphorylation (OXPHOS).
- While mitochondrial DNA mutations account for only 25% of pediatric cases and next-gen sequencing can be inconclusive, biochemical methods remain important for accurate diagnosis.
- The study introduced a method for isolating and cryopreserving peripheral blood mononuclear cells (PBMCs) from children's blood, achieving a 72% diagnosis confirmation rate in mitochondrial disease cases using high-resolution oxygraphy, though false negatives occurred in 13% of instances.
Poly(vinyl alcohol)/Chitosan Hydrogel Containing Gallic Acid-Modified Fe, Cu, and Zn Metal-Organic Frameworks (MOFs): Preparation, Characterization, and Biological Applications.
ACS Appl Mater Interfaces
November 2024
Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz 6135783151, Iran.
Hydrogel composites are water-swollen and three-dimensional materials that have been investigated for various biological applications, including controlled drug delivery and tissue engineering, owing to the similarity between their mechanical, electrical, and chemical properties with biological tissues. The hydrogel composites can provide a superior replication of living tissue compared to their single components. In this regard, Fe-BTC, Cu-BTC, and Zn-BTC MOFs were synthesized and modified with gallic acid (GA).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!