A total of 110 bulls coming from a farm where cysticercosis repeatedly occurred were examined by means of indirect haemagglutination (IHA), microprecipitation in agar (MPA), and indirect immunofluorescence reaction (IFR). Cysticercosis (isolated cysts with live or dead cysticerci) was detected in 29 of them at post-mortem examination. Among the 29 infected animals, positive reactions were obtained in 37.9% with IHA, 51.7% with MPA and 10.3% with IFR. The remaining animals were negative. Of 82 animals giving negative reactions with IHA, 22% harboured cysticerci at post-mortem examination. Of 70 animals negative with MPA, 20% were infected with cysticerci and of 71 animals negative with IFR, 29.5% were positive at the slaughter. Consequently, none of the used serological methods is reliable for the detection of a very light spontaneous infection.
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PLoS One
January 2025
Hongqi Hospital of Mudanjiang Medical University, Mudanjiang, Heilongjiang, China.
DNA methylation is known to be associated with cataracts. In this study, we used a mouse model and performed DNA methylation and transcriptome sequencing analyses to find epigenetic indicators for age-related cataracts (ARC). Anterior lens capsule membrane tissues from young and aged mice were analyzed by MethylRAD-seq to detect the genome-wide methylation of extracted DNA.
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Rollins School of Public Health, Emory University, 1518 Clifton Rd. NE Atlanta GA, Atlanta, 30322, USA.
This study aimed to explore the awareness, willingness, and engagement with pre-exposure prophylaxis (PrEP) among high-risk Chinese men who have sex with men (MSM) and to investigate the factors influencing its use. A cross-sectional survey of 1800 HIV-negative MSM was conducted in Chengdu, Suzhou, and Wuhan between June 2022 and February 2023 through in-person and online recruitment methods. Univariate and multivariate logistic regression analyses were used to identify predictors of PrEP use.
View Article and Find Full Text PDFBiochem Genet
January 2025
Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 50603, Kuala Lumpur, Malaysia.
Colorectal cancer (CRC) is the third most deadly cancer diagnosed in both men and women. 5-Fluorouracil (5-FU) treatment frequently causes the CRC cells to become chemoresistance, which has a negative impact on prognosis. Using bioinformatic techniques, this work describes important genes and biological pathways linked to 5-FU resistance in CRC cells.
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January 2025
Laboratory of Small Ruminant Genetics, Breeding and Reproduction, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Growth traits are one of the focuses of sheep breeding, and growth curve is an effective method to describe growth traits. The body weight of Yiling sheep at 0, 120, 180, 360 and 540 days of age were fitted with five common nonlinear growth models: Logistic, Gompertz, Von Bertalanffy, Brody and Negative exponential, and the growth models were evaluated by goodness of fit standard. The results showed that the Von Bertalanffy model was suitable for characterizing the growth of Yiling sheep.
View Article and Find Full Text PDFNucleic Acids Res
January 2025
Department of Chemistry and State Key Laboratory of Marine Pollution, City University of Hong Kong, Hong Kong SAR 999077, China.
RNA G-quadruplexes (rG4s) are non-canonical secondary nucleic acid structures found in the transcriptome. They play crucial roles in gene regulation by interacting with G4-binding proteins (G4BPs) in cells. rG4-G4BP complexes have been associated with human diseases, making them important targets for drug development.
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