The DKI vertical pipette puller (Model 700 C), which is normally used to make single or multi-barrel glass pipettes out of prepulled blanks, can be modified for the construction of a variety of multi-barrel pipettes. The use of the modified pipette puller is described for the production of small diameter 4-barrel pipettes.
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Different taste map for amiloride sensitivity, response frequency, and threshold to NaCl in the rostral nucleus of the solitary tract in rats.
Chem Senses
January 2024
Department of Physiology, School of Dentistry, Aichi-Gakuin University, Nagoya 464-8650, Japan.
Studies on taste bud cells and brain stem relay nuclei suggest that alternative pathways convey information regarding different taste qualities. Building on the hypothesis that amiloride (epithelial Na channel antagonist)-sensitive neurons respond to palatable salt (low-concentration) and amiloride-insensitive neurons respond to aversive salt (high-concentration), we investigated the histological distribution of taste-sensitive neurons in the rostral nucleus of the solitary tract in rats and their NaCl and amiloride sensitivities. We recorded neuronal activity in extracellular single units using multi-barrel glass micropipettes and reconstructed their locations on the rostrocaudal and mediolateral axes.
View Article and Find Full Text PDFDifferential effects of iontophoretic application of the GABAA-antagonists bicuculline and gabazine on tone-evoked local field potentials in primary auditory cortex: interaction with ketamine anesthesia.
Brain Res
July 2008
Institute for Neurobiology, University of Ulm, Albert-Einstein-Allee 11, Ulm, Germany.
gamma-Aminobutric acid (GABA) is one of the main inhibitory transmitters in the central nervous system. In a recent study we have demonstrated differential effects of two iontophoretically applied GABA(A)-blockers, bicuculline (BIC) and gabazine (SR 95531), on neuronal responses in primary auditory cortex (AI): Whereas the only effect of gabazine was to block GABA(A)-mediated inhibition, BIC application additionally induced dose-dependent side effects, probably on calcium-dependent potassium channels. Here we investigated the effects of the two drugs on pure tone-evoked local field potentials (LFPs) in AI.
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Department of Visual Science, University College London, UK.
1. The superficial grey layer of the superior colliculus (SGS) contains a high proportion of GABAergic inhibitory neurones. We have investigated the role of GABA receptors in synaptic transmission of aspects of visual activity in the SGS that may be driven by inhibitory mechanisms, such as surround inhibition and response habituation.
View Article and Find Full Text PDF[Effects of iontophoretically-applied LHRH, TRH, estrogen and clomiphene on thermo-sensitive neurons in the rat hypothalamus].
Nihon Sanka Fujinka Gakkai Zasshi
April 1985
Studies were performed on the correlation between the hypothalamic temperature-sensitive neurons and hypothalamic neurons sensitive to LHRH, TRH, conjugated estrogen and clomiphene in 103 castrated matured female rats by the technique of microiontophoresis using a multi-barrel glass microelectrode. Out of 348 neurons in the hypothalamus, 24 neurons were found to be LHRH-sensitive, 20 TRH-sensitive, 41 estrogen-sensitive, and 18 clomiphene-sensitive. There were 19(28.
View Article and Find Full Text PDFThe DKI vertical pipette puller (Model 700 C), which is normally used to make single or multi-barrel glass pipettes out of prepulled blanks, can be modified for the construction of a variety of multi-barrel pipettes. The use of the modified pipette puller is described for the production of small diameter 4-barrel pipettes.
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