AI Article Synopsis
- Researchers successfully created poly(2'-deoxy-2'-fluoroadenylic acid) [poly(Af)] from 2'-Deoxy-2'-fluoroadenosine through a polymerization process yielding 55%.
- The UV absorption and CD spectra of poly(Af) were similar to poly(A), indicating comparable structural properties, but poly(Af) showed lower melting temperatures (Tm) than poly(A).
- Poly(Af) formed specific complexes with poly(U) and poly(I), demonstrating its ability to interact similarly to poly(A) but distinct differences from poly(dA).
Article Abstract
2'-Deoxy-2'-fluoroadenosine was chemically transformed to its 5'-diphosphate and polymerized with polynucleotide phosphorylase to give poly(2'-deoxy-2'-fluoroadenylic acid) [poly(Af)]. Polymerization proceeded smoothly as in the case of poly(A) and the yield of the polymerization was 55%. The UV absorption spectra of poly(Af) closely resembled those of poly(A) and the hypochromicity was 32% at pH 7.0. The CD profile at 25 degrees and neutrality showed similar pattern to that of other poly(2'-deoxy-2'-halogenoadenylic acids) with somewhat larger [theta] values both in the positive and negative maxima. Acid titration of poly(Af) showed a transition point at pH 5.2 and the Tm of the acid form was 37 degrees which was significantly lower than that of poly(A), but similar to that of poly(2'-azido-2'-deoxyadenylic acid). Poly(Af) formed 1:1 and 1:2 complexes with poly-(U) having Tm of 49 degrees and 62 degrees at 0.04M and 0.15M Na(+) concentration, respectively. Poly(Af) also formed a 1:2 complex with poly(I) and its Tm was 36 degrees at 0.05M Na(+) concentration. These data showed that poly(Af) has rather similar properties to those of poly(A), but not to poly(dA).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC342131 | PMC |
| http://dx.doi.org/10.1093/nar/5.6.1877 | DOI Listing |
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