Pyridoxylated stroma-free hemoglobin solution was compared to hemodiluted blood in extremity perfusion of mongrel dogs. pH-values in tissue and in perfusate as well as oxygen tension showed no significant difference, and oxygen supply to the periphery was sufficient. Measurement of lactate, pyruvate, ATP and CP demonstrated domination of aerobic metabolism. We proved that stroma-free hemoglobin solution ( SFHb -PLP) can substitute hemodiluted blood (Hk 18) in isolated extremity perfusion. Metabolic results were confirmed by histological examination where no tissue alterations could be found in the perfused extremity or the whole body system.
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