The circulatory dynamics on the dorsal surface of the spinal cord were observed by serial fluorescein angiography . Fluorescein dye appeared in the posterior spinal artery and pial arterial plexus, although the direction of blood flow might be opposite and the time taken for fluorescein dye to enter the arteries was different for each. Fluorescein dye appeared in the veins after various periods, and the directions of flow of the dye were also variable and complicated. Laminar flow often was observed in veins of less than 200 microns in diameter. The regional circulation time was calculated to be 2.1 +/- 0.4 seconds. There was no extravasation.
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http://dx.doi.org/10.1097/00007632-198404000-00007 | DOI Listing |
Pathologica
October 2024
Biolab, PoliToBIOMed Lab, Department of Electronics and Telecommunications, Politecnico di Torino, Turin, Italy.
Objective: Stain normalization is a technique used to standardize the color appearance of digital whole slide images (WSIs). This study aimed to assess the impact of digital stain normalization on prostate cancer diagnosis by pathologists.
Methods: A multi-institutional board of four pathologists evaluated 407 hematoxylin and eosin (H&E) prostate WSIs before and after stain normalization.
Plant Foods Hum Nutr
December 2024
Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Periférico Norte Km. 33.5, Tablaje Catastral 13615, Col. Chuburná de Hidalgo Inn, Mérida, 97203, Yucatán, México.
The increasing concern over microbial resistance to conventional antimicrobial agents used in food preservation has led to growing interest in plant-derived antimicrobial peptides (AMPs) as alternative solutions. In this study, the antimicrobial mechanisms of chia seed-derived peptides YACLKVK, KLKKNL, KLLKKYL, and KKLLKI were investigated against Staphylococcus aureus (SA) and Escherichia coli (EC). Fluorometric assays and scanning electron microscopy (SEM) demonstrated that the peptides disrupt bacterial membranes, with propidium iodide (PI) uptake reaching 72.
View Article and Find Full Text PDFCNS Neurosci Ther
December 2024
Department of Anesthesiology, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.
Aims: This study investigated the protective role of Annexin A1 (ANXA1) in sepsis-associated encephalopathy (SAE) by examining its effects on brain vascular endothelium and blood-brain barrier (BBB) integrity.
Methods: Mice were divided into four groups: wild type (WT), cecal ligation and puncture (CLP), ANXA1 knockout (ANXA1[-/-]), and ANXA1(-/-) with CLP. Neurobehavioral changes were assessed using the Y-maze test, while BBB integrity was evaluated through Evans blue dye (EBD) staining and permeability tests with fluorescein isothiocyanate (FITC)-dextran.
Ophthalmol Sci
October 2024
Department of Biomedical Engineering, Stevens Institute of Technology, Hoboken, New Jersey.
Objective: To investigate retinal vascular permeability mapping as a potential biomarker for diabetic retinopathy in subjects with diabetes with no signs of retinopathy and with mild nonproliferative retinopathy.
Design: This is a case-control study.
Subjects: Participants included 7 healthy controls, 22 subjects with diabetes mellitus and no clinical signs of retinopathy (DMnoDR), and 7 subjects with mild nonproliferative diabetic retinopathy (NPDR).
J Fr Ophtalmol
December 2024
Biostatistics and Research Methodology Unit, School of Medical Sciences, Health Campus, University Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia.
Purpose: To correlate fluorescein dye disappearance test (FDDT) grades and endoscopic dye transit times (EDTT) in patients with patent osteotomies after nasolacrimal duct obstruction surgery (NLDO).
Design: Cross-sectional study.
Methods: All adult patients with patent osteotomies post-external dacryocystorhinostomy (Ex-DCR) or post-endoscopic dacryocystorhinostomy (EDCR) for NLDO were included in this cross-sectional investigation.
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