AI Article Synopsis
- - The study used molecular immunoelectron microscopy to analyze immune complexes of rabbit IgG and specific antibody fragments, revealing the number and location of allotypic epitopes (allotopes) on three different VH allotypes (a1, a2, a3).
- - Each VH allotype had at least two distinct allotopes, with their locations differing widely; some were found near the VH-CH1 switch region while others were near the complementarity-determining region.
- - The research also highlighted the rotational flexibility of the Fab arm of IgG, indicating that it can twist around its long axis, which contributes to the configurations formed during its interaction with the monoclonal antibody Fab.
Article Abstract
Immune complexes composed of rabbit IgG and Fab fragments of antibody specific for the VH framework allotypes were analyzed by molecular immunoelectron microscopy. In this manner, the number of allotypic epitopes ( allotopes ) and their approximate topological location could be determined. A monoclonal anti-allotype Fab was used to show that relatively fine details of allotope location and orientation are demonstrable with this technique. Each of the three VH allotypes (a1, a2 and a3) was found to express at least two spacially separated allotopes . The locations of the allotopes varied for each of the three allotypes. Some allotopes were observed near the VH-CH1 switch region while others were in close proximity to the complementarity-determining region or were at intermediate positions. In addition to the information concerning allotope topology, the variety of configurations resulting from the interaction of monoclonal antibody Fab with IgG suggest considerable rotational flexibility (twisting) of the Fab arm of IgG about its long axis.
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| http://dx.doi.org/10.1002/eji.1830140514 | DOI Listing |
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