This study compares the effects of passive administration of monoclonal anti-hapten (DNP) antibodies on primary plaque-forming cell (PFC) responses in mice to either soluble (DNP-keyhole limpet haemocyanin [KLH] ) or particulate (TNP-erythrocyte) antigens. IgM, IgG1, IgG2a and IgG2b antibodies at doses up to 500 micrograms induced at best a modest suppression of the IgM response, and reproducibly enhanced the IgG response to DNP-KLH by up to 30-fold. In contrast, with the particulate antigen only the IgM antibody enhanced IgG PFC; IgG2 antibodies, and one out of two IgG1 antibodies caused marked suppression of the primary response to TNP-RBC. This required antibody with an intact Fc portion. The enhancement of IgG responses to soluble antigen presumably reflects rapid B cell priming by immune complexes trapped by follicular dendritic cells in lymphoid follicles, in agreement with earlier data. These results indicate that the nature of the antigen can markedly influence the immunoregulatory effects of antibodies on humoral responses.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1454600 | PMC |
Publication Analysis
Top Keywords
Similar Publications
Isolation of Anti-Hapten Antibodies by Fluorescence-Activated Cell Sorting of Yeast-Displayed B-Cell Receptor Gene Repertoires.
Methods Mol Biol
December 2020
Antibody-Drug Conjugates and Targeted NBE Therapeutics, Merck KGaA, Darmstadt, Germany.
Anti-hapten antibodies are widely used as specific immunochemical detection tools in a variety of clinical and environmental analyses. The sensitivity, however, is limited due to the resulting antibody affinities to the haptens which, in turn, leads to a high demand for specific affinity reagents. A well-established path for the generation of high-affinity antibodies is the immunization of animals with the target antigen.
View Article and Find Full Text PDFChemically Programmed Bispecific Antibodies in Diabody Format.
J Biol Chem
September 2016
From the Departments of Cancer Biology, Molecular Therapeutics, The Scripps Research Institute, Jupiter, Florida 33458,
Chemically programmed bispecific antibodies (biAbs) endow target cell-binding small molecules with the ability to recruit and activate effector cells of the immune system. Here we report a platform of chemically programmed biAbs aimed at redirecting cytotoxic T cells to eliminate cancer cells. Two different antibody technologies were merged together to make a novel chemically programmed biAb.
View Article and Find Full Text PDFA new approach of indirect enzyme-linked immunosorbent assay for determination of D-glutamic acid through in situ conjugation.
J Immunoassay Immunochem
December 2016
a Graduate School of Pharmaceutical Sciences , Kyushu University, Higashi-ku , Fukuoka , Japan.
We propose a new approach of an indirect enzyme-linked immunosorbent assay (ELISA) for determination of D-glutamic acid (D-Glu) using a monoclonal antibody against D-glutamic acid (D-Glu-MAb), which recognizes D-Glu-glutaraldehyde (GA) molecule but not D-Glu molecule. Human serum albumin (HSA) was coated on an immunoplate and reacted with D-Glu via GA to produce D-Glu-GA-HSA conjugates in situ in the well to be recognized by D-Glu-MAb, which enabled the development of an indirect ELISA for the determination of free D-Glu. In this indirect ELISA, D-Glu can be specifically detected with limit of detection of 7.
View Article and Find Full Text PDFDevelopment of an imaging-guided CEA-pretargeted radionuclide treatment of advanced colorectal cancer: first clinical results.
Br J Cancer
August 2013
Radboud University Nijmegen Medical Centre, PO Box 9101, Nijmegen, The Netherlands.
Background: Radiolabelled antibody targeting of cancer is limited by slow blood clearance. Pretargeting with a non-radiolabelled bispecific monoclonal antibody (bsMAb) followed by a rapidly clearing radiolabelled hapten peptide improves tumour localisation. The primary goals of this first pretargeting study in patients with the anti-CEACAM5 × anti-hapten (HSG) bsMAb, TF2, and the radiolabelled hapten-peptide, IMP288, were to assess optimal pretargeting conditions and safety in patients with metastatic colorectal cancer (CRC).
View Article and Find Full Text PDFPretargeted immuno-PET of CEA-expressing intraperitoneal human colonic tumor xenografts: a new sensitive detection method.
EJNMMI Res
January 2012
Dept, of Nuclear Medicine, Radboud University Nijmegen Medical Centre, 6500 HB, Nijmegen, 9101, The Netherlands.
Background: In this study, pretargeted immuno-positron-emission tomography [PET] with a bispecific monoclonal anti-carcinoembryonic antigen [CEA] (CEACAM5) × anti-hapten antibody (bispecific monoclonal antibody [bsmAb]) and a small (1.5 kD) peptide labeled with 68Ga was compared to fludeoxyglucose [18F-FDG]-PET for detecting intraperitoneal [i.p.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!