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Clearing the path for whole-mount labeling and quantification of neuron- and vessel-density in adipose tissue.
J Cell Sci
January 2025
Institute of Molecular Biosciences, University of Graz, Graz, Austria.
White adipose tissue (WAT) comprises a plethora of cell types beyond adipocytes forming a regulatory network that ensures systemic energy homeostasis. Intertissue communication is facilitated by metabolites and signaling molecules that are spread by vasculature and nerves. Previous works indicated that WAT responds to environmental cues by adapting the abundance of these "communication routes", however, high intra-tissue heterogeneity questions the informative value of bulk or single cell analyses and underscores the necessity of whole-mount imaging.
View Article and Find Full Text PDFA non-derivatized high-performance liquid chromatography method for simultaneous quantification of hydroxyl acids and amino acids in ammonolysis reactions.
Anal Methods
January 2025
School of Food and Bioengineering, Changsha University of Science & Technology, Changsha, Hunan, 4100114, China.
A non-derivatized high-performance liquid chromatographic (HPLC) method was developed for the simultaneous quantification of hydroxyl acids and their amination products in ammonolysis reaction mixtures. By optimizing the mobile phase composition and pH (0.04 M KHPO-5% methanol, pH = 2.
View Article and Find Full Text PDFDetermination of 115 Pesticide Residues in Textiles by Liquid Chromatography-Tandem Mass Spectrometry.
Rapid Commun Mass Spectrom
May 2025
Technology Center of Qingdao Customs, Qingdao, China.
The presence of pesticide residues in textiles poses a risk to human health. We established a robust and high-throughput liquid chromatography-tandem mass spectrometry method for the determination of 115 pesticide residues in textiles. In this study, we evaluated high-performance liquid chromatography-tandem mass spectrometry conditions and sample extraction methods, including separation performance of different columns, mass conditions, extraction solvent, and extraction time.
View Article and Find Full Text PDFEvaluation of Bioactive Compounds and Chemical Elements in Herbs: Effectiveness of Choline Chloride-Based Deep Eutectic Solvents in Ultrasound-Assisted Extraction.
Molecules
January 2025
Department of Food Plant Chemistry and Processing, Faculty of Food Science, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland.
In this study, the effectiveness of three choline chloride (ChCl)-based deep eutectic solvents (DESs) formed using malonic acid (MalA), glycerol (Gly), and glucose (Glu) as hydrogen bond donors and two conventional solvents (50% methanol and 50% ethanol) for ultrasonic-assisted extraction (UAE) of antioxidant compounds from four herbs (chamomile, lemon balm, nettle, and spearmint) were estimated. The antioxidant capacity (AC) of the obtained herb extracts was determined by the modified 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and cupric reducing antioxidant capacity (CUPRAC) methods. Profiles of phenolic acids, flavonoid aglycones, and flavonoid glycosides in the green and conventional herb extracts were quantitatively analyzed using ultra-performance liquid chromatography (UPLC).
View Article and Find Full Text PDFHigh-Sensitivity Fluorescent Sensing Platform for Rapid and Selective Detection of Chlorpyrifos Pesticide in Water Samples Using Terbium-Isatin-3-allyl Complex.
J Fluoresc
January 2025
Department of Chemistry, Faculty of Science, Suez Canal University, Ismailia, 41522, Egypt.
The detection of organophosphorus pesticides, particularly chlorpyrifos, in environmental samples is essential due to their widespread use and associated health risks. In this study, we developed a high-sensitivity fluorescent sensing platform utilizing an Isatin-3-allyl-terbium (IS-Tb) complex in solution for the rapid and selective detection of chlorpyrifos in various water samples. The proposed chemical structure of the complex in solution was evaluated using molar ratio method.
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