Prematurely condensed chromosomes (PCC) have been obtained by polyethylene glycol (PEG) induced fusion in suspension of the Chinese hamster metaphase cultured cells with those in interphase. As alternative approach the PEG-fusion of the Chinese hamster asynchronous culture cells in monolayer with subsequent incubation in free medium was used. A comparative cytofluorimetric investigation of PCC and chromatin of the interphase nuclei of corresponding ploidy has shown some increase (up to 10%) of acridine orange and olivomycin binding with PCC chromatin. A similar slight increase in low molecular weight ligands binding with chromatin was also found in mitotic chromosomes. The data obtained confirm the opinion about the similarity of events taking place in chromatin during physiological mitosis and premature chromosome condensation. The cytochemical study of chromatin availability to low molecular weight ligands can be used as a criterion for judging on the properties of the artificially condensed chromatin.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Measuring age-dependent viscoelasticity of organelles, cells and organisms with time-shared optical tweezer microrheology.
Nat Nanotechnol
January 2025
ICFO-Institut de Ciències Fotòniques, Castelldefels, The Barcelona Institute of Science and Technology, Barcelona, Spain.
Quantifying the mechanical response of the biological milieu (such as the cell's interior) and complex fluids (such as biomolecular condensates) would enable a better understanding of cellular differentiation and aging and accelerate drug discovery. Here we present time-shared optical tweezer microrheology to determine the frequency- and age-dependent viscoelastic properties of biological materials. Our approach involves splitting a single laser beam into two near-instantaneous time-shared optical traps to carry out simultaneous force and displacement measurements and quantify the mechanical properties ranging from millipascals to kilopascals across five decades of frequency.
View Article and Find Full Text PDFFreeze-Induced Protein Assembly of α-Synuclein into Stable Microspheres to Fabricate Light-Induced Cargo Release Systems.
ACS Appl Mater Interfaces
January 2025
School of Chemical and Biological Engineering, Institute of Engineering Research, College of Engineering, Seoul National University, Seoul 08826, Republic of Korea.
Stable hollow-type microspheres (MSs) have been fabricated using α-synuclein (αS), an amyloidogenic protein, via freeze-induced protein self-assembly. This assembly process involves three steps: rapid freezing to form spherical protein condensates from αS oligomers, frozen annealing to form a crust on the condensate and freeze-drying to create an interior lumen via the three-dimensional (3D) coffee-stain effect. The crust produced during the frozen-annealing step is a β-sheet-mediated protein structure that is presumed to be created at the quasi-liquid layer of the protein-ice interface and thus contributes to the stability of MSs in aqueous solutions at room temperature without any additional surface stabilization.
View Article and Find Full Text PDFMolecular Mechanisms of Aerosol Nucleation: from CLOUD Chamber Experiments to Field Observations.
Chimia (Aarau)
November 2024
Center for Energy and Environment, Paul Scherrer Institute, Villigen, CH-5232 Villigen, Switzerland.
Article Synopsis
- Atmospheric aerosol particles cause over four million premature deaths each year and significantly impact Earth's climate.
- Most atmospheric particles and over 50% of cloud condensation nuclei are created through new particle formation, involving specific precursor vapors.
- Experiments conducted at the CERN CLOUD chamber reveal the importance of sulfuric acid, highly oxygenated organic molecules, iodine oxoacids, and stabilizers like ammonia in this process, enhancing our understanding of atmospheric conditions and improving models for air quality and climate.
NeoVault: empowering neonatal research through a neonate data hub.
BMC Pediatr
November 2024
Department of Condensed Matter Physics, University of Cádiz, Cádiz, 11510, Spain.
Background: Stability during early postnatal life in preterm infants is related to better outcomes. Although vital signs are monitored continuously in Neonatal Intensive Care Unites, this monitoring does not include all physiological parameters nor data such as movement patterns. Although there are scattered sources of data, there is no centralized data hub for neonates information.
View Article and Find Full Text PDFEarly and late postnatal lung distribution of collagen type VI in preterm and term infants.
Respir Physiol Neurobiol
January 2025
Department of Women's and Children's Health, Uppsala University, Uppsala SE-751 85, Sweden.
Collagen type VI (COL6) is an important component of the extracellular matrix (EM) and may have a major role in lung development and disease. Studies on COL6 expression during lung development are mainly based on animal models. The aim of the study was to define COL6 expression pattern in lung parenchyma in infants with different lung maturational stages.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!