Micrococcal nuclease used to digest nuclei remains bound to the chromatin in an inactive form. The enzyme activity can be restored in situ by addition of divalent cations such as Ca++ or Mg++ resulting in continued digestion of high molecular weight chromatin into smaller fragments as demonstrated by two dimensional gel electrophoresis of samples as chromatin and DNA in the first and second dimensions, respectively. The bound enzyme can be selectively removed from the chromatin by treatment with cation exchange resin AG 50 WX2 at low salt concentration without altering the electrophoretic mobility of the chromatin.
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http://dx.doi.org/10.1093/nar/12.3.1391 | DOI Listing |
J Agric Food Chem
January 2025
College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China.
() is the primary risk factor in food safety. Herein, a nanogap-assisted surface-enhanced Raman scattering/polymerase chain reaction (SERS/PCR) biosensor coupled with a machine-learning tool was developed for the direct and specific sensing of S. aureus in milk.
View Article and Find Full Text PDFMethods Mol Biol
January 2025
Department of Biomedical Sciences, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND, USA.
Hox genes play a pivotal role during development. Their expression is tightly controlled in a spatiotemporal manner, ensuring that specific body structures develop at the correct locations and times during development. Various genomics approaches have been used to capture temporal and dynamic regulation of Hox gene expression at the nucleosome/chromatin level.
View Article and Find Full Text PDFGeorgian Med News
October 2024
1College of Education, Al-Iraqia University, Baghdad, Iraq.
Background: During this study, six isolates of multiple antibiotic resistant Staphylococcus aureus bacteria were obtained from different clinical specimens (burn swabs, urinary tract infections, wound swabs): three isolates from burns, two isolates from urinary tract infections, and one isolate from wound swabs. They were obtained from private laboratories in Baghdad from 1/1/2023 to 3/15/2023.
Method: The diagnosis of these isolates was confirmed using the Vitek2 device.
Int J Food Microbiol
January 2025
School of Life Sciences, Anhui Agricultural University, Hefei, Anhui 230036, China; Food Procession Research Institude, Anhui Agricultural University, Hefei, Anhui 230036, China. Electronic address:
Staphylococcus aureus (S. aureus) is a primary culprit of food poisoning. As a highly adaptable pathogen, S.
View Article and Find Full Text PDFJ Biol Chem
November 2024
RNAcious Laboratory, Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland. Electronic address:
Nucleases, that is, enzymes that catalyze the hydrolysis of phosphodiester bonds in nucleic acids, are essential tools in molecular biology and biotechnology. Staphylococcus aureus nuclease is particularly interesting due to its thermostability and Ca dependence, making it the prime choice for applications where nuclease modulation is critical, such as ribosome profiling in bacteria and halophilic archaea. The latter poses a technical and economical challenge: high salt reaction conditions are essential for maintaining ribosome integrity but negatively impact the micrococcal nuclease (MNase) activity, necessitating using large amounts of nuclease to achieve efficient cleavage.
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