Peritoneal exudate was collected from rabbits 18 hr after ip injection of shellfish glycogen. The fluid was centrifuged and the peritoneal exudate supernatant (PES) retained for use in growth studies. The growth of Pasteurella multocida in PES was inhibited at 41 degrees C (febrile temperature for rabbits) as compared with 39 degrees C (afebrile temperature), suggesting the presence of an antibacterial agent active at febrile temperature. Further studies indicated that this antibacterial substance has a molecular weight less than 5000 Da. Heat treatment (70 degrees C, 1 hr) had no influence upon the activity of the inhibitory factor.
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