Using DNA sedimentation in alkaline sucrose gradients, the excision of pyrimidine dimers from DNA of CHO cells with the help of UV-endonuclease from Micrococcus luteus has been studied. A considerable proportion (approximately 80%) of pyrimidine dimers was shown to be excised within 26 hours, whereas during the initial 17 hours after the UV-fluence (4 J/M2) the excision was slight (approximately 15%).
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