Fluorescence labeled erythrocytes are suitable markers for red cell velocity and red cell flux through glomerular capillaries in incident light fluorescence microscopy. Mean velocity of the labeled cells was measured from a video recording with a microprocessor using a modified dual slit photometric technic. Red cell flux through the capillary was derived from the labeled cell frequency in capillary flow and systemic concentration of the fluorescent cells. The capillary diameters could be determined after staining plasma with fluorescence labeled dextran. The dynamic hematocrit (HMICRO) was calculated by the ratio of red cell flux to the product of red cell velocity and luminal cross section and varied between +28 and -51% from the corresponding systemic value.
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Eur J Med Res
January 2025
Division of Radiology, Saraburi Hospital, Saraburi, Thailand.
Introduction: Stroke-associated pneumonia (SAP) is a major cause of mortality during the acute phase of stroke. The ADS score is widely used to predict SAP risk but does not include 24-h non-contrast computed tomography-Alberta Stroke Program Early CT Score (NCCT-ASPECTS) or red cell distribution width (RDW). We aim to evaluate the added prognostic value of incorporating 24-h NCCT-ASPECTS and RDW into the ADS score and to develop a novel prediction model for SAP following thrombolysis.
View Article and Find Full Text PDFNat Commun
January 2025
Unidade de Xenética, Instituto de Ciencias Forenses, Facultade de Medicina, Universidade de Santiago de Compostela, 15782, Calle San Francisco sn, Galicia, Spain.
Mycoplasma pneumoniae causes atypical pneumonia in children and young adults. Its lack of a cell wall makes it resistant to beta-lactams, which are the first-line treatment for typical pneumonia. Current diagnostic tests are time-consuming and have low specificity, leading clinicians to administer empirical antibiotics.
View Article and Find Full Text PDFMedicine (Baltimore)
November 2024
Department of Clinical Pharmacy, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Background: Patients with hematological malignancies are at high-risk of Clostridium difficile infection (CDI). Oral vancomycin is a first-line treatment for CDI. Vancomycin has been widely reported to induce flushing syndrome (also known as Red man syndrome), a well-known hypersensitivity reaction mostly occurs after intravenous administration.
View Article and Find Full Text PDFTalanta
January 2025
Key Laboratory for Advanced Materials, Joint International Research Laboratory for Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, PR China. Electronic address:
Hypochlorous acid (HClO/ClO) is a common ROS that exhibits elevated activity levels in cancer cells. In this study, an ClO-triggered TADF probe, PTZ-MNI, was designed based on a naphthalimide core. PTZ-MNI self-assemble in aqueous environments, exhibiting significantly enhanced fluorescence that demonstrated typical aggregation-induced delayed fluorescence (AIDF) characteristics.
View Article and Find Full Text PDFJ Magn Reson
January 2025
Center for Pulmonary Imaging Research (CPIR), Division of Pulmonary Medicine Cincinnati Children's Hospital Medical Center Cincinnati OH USA; Department of Pediatrics, University of Cincinnati OH USA; Department of Biomedical Engineering, University of Cincinnati OH USA; Imaging Research Center (IRC), Department of Radiology Cincinnati Children's Hospital Medical Center Cincinnati OH USA. Electronic address:
Harmonizing and validating Xe gas exchange imaging across multiple sites is hampered by a lack of a quantitative standard that 1) displays the unique spectral properties of Xe observed from human subjects in vivo and 2) has short enough T times to enable practical imaging. This work describes and demonstrates the development of two dissolved-phase, thermally polarized phantoms that mimic the in-vivo, red blood cell and membrane resonances of Xe dissolved in human lungs. Following optimization, combinations of two common organic solvents, acetone and dimethyl sulfoxide, resulted in two in-vivo-like dissolved-phase Xe phantoms yielding chemical shifts of 212.
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