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Evaluating diagnostic performance: A comparative analysis of cell-free DNA and serological test in hepatic cystic Echinococcosis.
J Helminthol
January 2025
Hacettepe University, Faculty of Medicine, Department of Radiology, Ankara, Turkiye.
Cystic Echinococcosis (CE) is a zoonotic disease caused by sensu lato. Diagnosing CE primarily relies on imaging techniques, and there is a crucial need for an objective laboratory test to enhance the diagnostic process. Today, cell-free DNAs (cfDNAs) have gained importance regarding their biomarker potential.
View Article and Find Full Text PDFELISA based seroprevalence and risk factors for three zoonotic parasites (Toxoplasma gondii, Echinococcus granulosus and Trichinella spiralis) in humans in Khyber Pakhtunkhwa, Pakistan.
Parasitol Int
December 2024
Department of Veterinary Pathobiology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX, USA. Electronic address:
Zoonotic parasitic infections can affect both animal and human health, while also having food safety and economic implications. These pathogens can also pose occupational risks for certain groups, including veterinary professionals, animal handlers, and butchers. This study was conducted in the district of Swabi located in the Khyber Pakhtunkhwa Province of northwestern Pakistan.
View Article and Find Full Text PDFAdvances in Detecting Cystic Echinococcosis in Intermediate Hosts and New Diagnostic Tools: A Literature Review.
Vet Sci
May 2024
Medical Graduated Student, Medical School, Shahid Bahonar University of Medical Sciences, Kerman 7618411764, Iran.
Cystic echinococcosis (CE) is a zoonotic disease affecting humans and animals. Despite a lack of clarity about many details of parasite-intermediate host interactions, the nature of the immune responses triggered by hydatid infection has revealed new perspectives. This study discusses the latest advances in elucidating the immunologic mechanism of echinococcosis and its detection and potential approaches to enhance serodiagnosis accuracy.
View Article and Find Full Text PDFHuman and camel cystic echinococcosis - a polyclonal antibody-based sandwich ELISA for its serodiagnosis with molecular identification.
Vet Res Commun
August 2024
Hepato-Gastroenterology and Infectious Diseases Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
Article Synopsis
- - Cystic echinococcosis (CE) is a neglected disease prevalent in Egypt, affecting both humans and camels; this study assessed a new diagnostic method using a specific test to identify Echinococcus granulosus antigens in their blood.
- - Researchers obtained antigens from infected camels and developed polyclonal antibodies by immunizing rabbits, which were then purified and tested for effectiveness using a sandwich ELISA method, showing excellent sensitivity and specificity.
- - The sandwich ELISA revealed that 33.3% of human and 55.6% of camel samples tested positive for CE, demonstrating the method's potential for accurate diagnosis without cross-reactivity to other diseases.
Comparison of Methods in the Serologic Diagnosis of Cystic Echinococcosis.
Acta Parasitol
June 2024
Department of Medical Microbiology, School of Medicine, Gazi University, 06490, Ankara, Türkiye.
Purpose: Cystic echinococcosis (CE) is caused by the larval form of Echinococcus granulosus. Clinical, radiologic, pathologic, and serologic findings should be evaluated together for the diagnosis of CE. The sensitivity and specificity oalf serologic tests may vary depending on the method used.
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