Phosphatidyl ethanolamine methylation in membrane from bronchoalveolar lavage mononuclear cells, in asthmatic patients: a new marker of macrophage activity.

Phosphatidyl ethanolamine methylation was compared in alveolar macrophage membrane from asthmatic and control subjects. Phosphatidyl ethanolamine methylase activity was determined by measuring the incorporation of (3H) methyl group from (3H) adenosyl methionine into membrane phospholipids. (3H) methyl group incorporation was significantly higher in macrophages from asthmatic patients. This result is consistent with macrophage membrane activation and could signify: a membrane phospholipid pool regeneration after allergenic or toxic disturbance or an enzymatic activation by inflammatory mediators.