[Effect of ultra low temperatures on the acrosomal proteins of bull and ram spermatozoa].

Tested was the effect in vitro of ultra low temperatures on the activity of the acrosomal enzymes--acrosine and acid phosphatase--in the spermatozoa of bulls and rams. The use of spectrophotometric and electrophoretic methods demonstrated the presence of certain differences in the electrophoretic profile of the studied enzymes so far as spermatozoa of bulls and rams were concerned. Besides, acrosine and acid phosphatase in the acrosomal extracts from ram spermatozoa showed higher lability in treatment with ultra low temperatures as compared to bull spermatozoa. This led to higher losses with regard to some biologic properties of ram spermatozoa following the use of a technology of deep-freezing of semen.