A simple and reliable method has been developed for the detection of benzoylecgonine in urine by UV spectrophotometry. Benzoylecgonine was isolated by liquid-liquid extraction and subsequently identified based on its hydrolysis by NaOH. The decrease in absorbance at 235 nm caused by this NaOH hydrolysis was used to identify benzoylecgonine even in the absence of its characteristic spectrum. None of the other drugs evaluated interfered with the method, which has a detection limit of 1.25 micrograms/mL.
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| http://dx.doi.org/10.1093/jat/7.5.235 | DOI Listing |
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