The results of the study of concentrated, purified, UV-inactivated cell-culture rabies vaccine, obtained from strain Vnukovo-22, passage 33-40, in the primary culture of Syrian hamster kidney cells, demonstrated the pronounced antigenic potency of this vaccine: when introduced intramuscularly in 3-4 injections at certain intervals, it induced the production of virus-neutralizing antibodies in high titers. In tests on volunteers the vaccine proved to be nonreactogenic.
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Adv Biotechnol (Singap)
February 2024
School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China.
While biotechnologies offer eco-friendly solutions for eliminating air contaminants, there is a scarcity of research examining the impacts of microbial purification of air pollutants on the structure and function of air microbial communities. In this study, we explored a Lactobacillus paracasei B1 (LAB) agent for removing ammoniacal odour. The impacts of LAB on air bacterial community were revealed.
View Article and Find Full Text PDFJ Bacteriol
January 2025
Interfaculty Institute of Microbiology and Infection Medicine Tübingen (IMIT), Department of Microbiology and Biotechnology, University of Tübingen, Tübingen, Baden-Württemberg, Germany.
Unlabelled: is well adapted to survive and persist in the infected host, escaping the host's immune response. Since polyamines such as spermine, which are synthesized by infected macrophages, are able to inhibit the growth of , the pathogen needs strategies to cope with these toxic metabolites. The actinomycete , a close relative of makes use of a gamma-glutamylation pathway to functionally neutralize spermine.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
Department of Microbiology, University of Delhi South Campus, New Delhi 110021, India. Electronic address:
The SUMO fusion technology has immensely contributed to the soluble production of therapeutics and other recombinant proteins in E. coli. The structure-based functionality of SUMO protease has remained the primary determinant for choosing SUMO as a solubility enhancer tag.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China. Electronic address:
A heteropolysaccharide, designated JYP70-1, was extracted and purified from the fruits of Rosa laevigata, exhibiting a molecular weight of 1.90 × 10 g/mol. Structural analysis revealed that JYP70-1 was composed of eleven sugar residues, including α-l-Araf-(1→, →3)-α-l-Araf-(1→, →5)-α-l-Araf-(1→, →3,5)-α-l-Araf-(1→, →2,5)-α-l-Araf-(1→, →4)-α-d-Galp-(1→, →6)-β-d-Galp-(1→, →6)-α-d-Glcp-(1→, α-d-Glcp-(1→, →2)-α-d-Manp-(1→, and →3,6)-β-d-Manp-(1→.
View Article and Find Full Text PDFPoult Sci
January 2025
College of Veterinary Medicine, Anhui Agricultural University, 130 West Changjiang Road, Hefei, Anhui 230036, China. Electronic address:
Chicken surfactant protein A1 (cSP-A1) is a soluble C-type lectin found primarily in chicken lungs. Its function and other potential bioactivities are unclear. This study aimed to express, purify, and identify recombinant cSP-A1 (RcSP-A1), investigate its effects on chicken macrophage HD11 cells, and evaluate its ability to regulate the LPS-induced inflammatory response.
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