Diamide, directly added to human erythrocytes, inhibits glucose utilisation. The trend of this process is in good correlation with the intracellular concentration of GSH. Since diamide does not affect the glycolytic and hexose monophosphate-shunt-pathway enzymes, it is likely that the effect of diamide involves the sugar transport across erythrocyte membrane. Kinetic studies carried out on ghosts have shown that diamide decreases Vmax without affecting Km. GSH on the contrary stimulates glucose transport by increasing Vmax. The significance of GSH in the process of transport of sugar is discussed.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Introduction: Varicella-Zoster virus (VZV) is a highly contagious alpha-herpes virus. The diagnosis of chickenpox remains a difficult task especially in cases of breakthrough chickenpox, so the development of reliable laboratory tests is necessary. The simplest and most sensitive serological test for detecting antibodies in human and animal sera is the passive hemagglutination reaction (PHAR).
View Article and Find Full Text PDFRed cell distribution width/platelet ratio predicts decompensation of metabolic dysfunction-associated steatotic liver disease-related compensated advanced chronic liver disease.
World J Gastroenterol
January 2025
Department of Internal Medicine, Azienda Ospedaliero-Universitaria of Modena (2023), Modena 41126, Italy.
Prognostication of compensated advanced chronic liver disease (cACLD) is of paramount importance for the physician-and-patient communication and for rational clinical decisions. The paper published by Dallio reports on red cell distribution width (RDW)/platelet ratio (RPR) as a non-invasive biomarker in predicting decompensation of metabolic dysfunction-associated steatotic liver disease (MASLD)-related cACLD. Differently from other biomarkers and algorithms, RPR is inexpensive and widely available, based on parameters which are included in a complete blood count.
View Article and Find Full Text PDFAbnormally Low HbA Caused by Hemolytic Anemia, a Case Report and Literature Review.
Br J Biomed Sci
January 2025
Viollier AG, Allschwil, Switzerland.
Hemoglobin A is a widely used diagnostic tool for monitoring glycemic control in diabetes management. However, its accuracy can be influenced by various factors. We present a case of a 17-year-old boy with abnormally low Hemoglobin A levels caused by warm autoantibody-induced hemolytic anemia.
View Article and Find Full Text PDFQuantifying glucose uptake at the single cell level with confocal microscopy reveals significant variability within and across individuals.
Sci Rep
January 2025
Applied Research and Technology, Abbott Diagnostics Division, Abbott Laboratories, Abbott Park, IL, 60064, USA.
Measurement of glycated hemoglobin (HbA1c) in human red blood cells plays a critical role in the diagnosis and treatment of diabetes mellitus. However, recent studies have suggested large variation in the relationship between average glucose levels and HbA1c, creating the need to understand glucose variability at the cellular level. Here, we devised a fluorescence-based method to quantitatively observe GLUT1-mediated intracellular glucose analog tracer uptake in individual RBCs utilizing microfluidics and confocal microscopy.
View Article and Find Full Text PDFIn vivo vessel connection of pre-vascularised 3D-bioprinted gingival connective tissue substitutes.
Biofabrication
January 2025
Univ. Bordeaux, INSERM U1026 (BioTis), CHU Bordeaux, Université de Bordeaux Collège Sciences de la Santé, 146 Rue Léo Saignat, Bordeaux, 33000, FRANCE.
Producing oral soft tissues using tissue engineering could compensate for the disadvantages of autologous grafts (limited availability and increased patient morbidity) and currently available substitutes (shrinkage). However, there is a lack of in vitro-engineered oral tissues due to the difficulty of obtaining stable pre-vessels that connect to the host and enable graft success. The main objective was to assess the connection of pre-vascularised 3D-bioprinted gingival substitutes to the host vasculature when subcutaneously implanted in immunodeficient mice.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!