Investigations were carried out to establish the effect of deep freezing, extent of dilution, and site of applying the spermatozoa as well as the functional state of the female genital apparatus of sheep on the forward movement of spermatozoa. It was found that at the insemination of ewes with freshly obtained undiluted semen at the 30th min following insemination there were single live spermatozoa, in the oviducts their number gradually increased up to the fourth hour, reaching several thousand. The highly diluted semen was found to produce an unfavourable effect on the movement of spermatozoa. Following deep freezing spermatozoa manifested a sharp decrease in their movement capacity. Deep cervical application led to an increased number of spermatozoa reaching the oviducts, however, this number was essentially lower than with freshly obtained semen. The movement of spermatozoa depended also on the functional status of the female sexual apparatus. There were considerably more spermatozoa in that of the oviducts on the side of which ovulation took place.
Download full-text PDF |
Source |
---|
Pharmaceuticals (Basel)
December 2024
Department of Biology, Chungnam National University, Daejeon 34134, Republic of Korea.
Objectives: The present study describes the comparative effect of 24-week supplementation of beeswax alcohol (BWA, Raydel, 0.5% and 1.0%, wt/wt) and coenzyme Q (CoQ, 0.
View Article and Find Full Text PDFGenes (Basel)
January 2025
Institute for Regenerative Medicine and Biotherapy (IRMB), University of Montpellier, INSERM, CHU Montpellier, 34295 Montpellier, France.
Background: Sperm samples are separated into bad and good quality samples in function of their phenotype, but this does not indicate their genetic quality.
Methods: Here, we used GeneChip miRNA arrays to analyze microRNA expression in ten semen samples selected based on high-magnification morphology (score 6 vs. score 0) to identify miRNAs linked to sperm phenotype.
Theriogenology
January 2025
Veterinary Clinic for Reproductive Medicine and Neonatology, Justus Liebig-University of Giessen, Germany.
Sperm cryopreservation is crucial in reproductive biotechnology; however, the longevity of frozen and thawed semen is limited by the deterioration of sperm cell integrity. This study aimed to examine the effects of adding antifreeze protein III (AFP III) to the diluent, using samples from eight healthy mature dogs. The ejaculates were divided into aliquots and diluted with a standard Tris-fructose-egg yolk extender containing AFP III at concentrations of 0, 0.
View Article and Find Full Text PDFHum Reprod
January 2025
IRSD, Université de Toulouse, INSERM, INRAE, ENVT, Univ Toulouse III-Paul Sabatier (UPS), Toulouse, France.
Study Question: Does a human fallopian tube (HFT) organoid model offer a favourable apical environment for human sperm survival and motility?
Summary Answer: After differentiation, the apical compartment of a new HFT organoid model provides a favourable environment for sperm motility, which is better than commercial media.
What Is Known Already: HFTs are the site of major events that are crucial for achieving an ongoing pregnancy, such as gamete survival and competence, fertilization steps, and preimplantation embryo development. In order to better understand the tubal physiology and tubal factors involved in these reproductive functions, and to improve still suboptimal in vitro conditions for gamete preparation and embryo culture during IVF, we sought to develop an HFT organoid model from isolated adult stem cells to allow spermatozoa co-culture in the apical compartment.
Zhongguo Zhen Jiu
January 2025
College of Acupuncture-Moxibustion and Tuina, Chengdu University of TCM, Chengdu 610075, Sichuan Province, China; Second Clinical College, Shanxi University of CM, Jinzhong 030619.
Objective: To observe the effects of the "Zhibian" (BL54)-toward-"Shuidao" (ST28) acupuncture on key regulatory factors during mitochondrial apoptosis of testicular tissue in asthenozoospermia mice, and explore the potential mechanism of the protective effect of acupuncture on reproductive function.
Methods: Thirty C57BL/6 male mice were randomly divided into a blank group, a model group and an acupuncture group, 10 mice in each group. In the model and the acupuncture groups, the intraperitoneal injection of cyclophosphamide (30 mg•kg•d) was delivered for 7 days to prepare the asthenozoospermia model.
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!