In this paper an analytical colorimetric method is presented where 2,4-dinitrophenyl hydrazine is used to measure small amounts of succinic semialdehyde in the presence of 2-oxoglutarate. This method is applicable to the measurement of 4-aminobutyrate-2-oxoglutarate aminotransferase activity, where succinic semialdehyde formed during the enzymatic reaction has to be measured in the presence of 2-oxoglutarate.
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