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Gel-Based Sample Fractionation with SP3-Purification for Top-Down Proteomics.
J Proteome Res
January 2025
Advanced Research Support Center, Ehime University, Ehime 791-0295, Japan.
Precise prefractionation of proteome samples is a potent method for realizing in-depth analysis in top-down proteomics. PEPPI-MS (Passively Eluting Proteins from Polyacrylamide gels as Intact species for MS), a gel-based sample fractionation method, enables high-resolution proteome fractionation based on molecular weight by highly efficient extraction of proteins from polyacrylamide gels after SDS-PAGE separation. Thereafter it is essential to effectively remove contaminants such as CBB and SDS from the PEPPI fraction prior to mass spectrometry.
View Article and Find Full Text PDFInterplay between the isotherm course and the efficiency of mAb purification in flowthrough and bind-and-elute modes on cation exchange resins.
J Chromatogr A
January 2025
Department of Chemical and Process Engineering, Rzeszów University of Technology, Rzeszów/PL. Electronic address:
Separation of a monoclonal antibody (mAb) from impurities was examined on different cation exchange resins (CEX), including POROS XS, POROS HS, NUVIA S, and NUVIA HRS. Impurities mainly consisted of cell culture-derived mAb fragments, or lysozyme, that mimicked the presence of an adsorbing protein of low molecular weight. The choice between the flowthrough mode and the bind-and-elute mode for the purification was guided by the shape of the adsorption isotherm.
View Article and Find Full Text PDFBiosurfactants: A review of different strategies for economical production, their applications and recent advancements.
Adv Colloid Interface Sci
January 2025
Department of Biotechnology, School of Applied Sciences and Technology, BLDE (Deemed to be University), Bangaramma Sajjan Campus, Vijayapura 586103, India; Department of Basic Sciences, Faculty of Engineering and Technology, CMR University, Bangalore 562149, India. Electronic address:
Biosurfactants are biodegradable, non-toxic, and environmentally beneficial substances that are produced by microorganisms. Due to their chemical characteristics and stability in various environmental circumstances, biosurfactants are low-molecular-weight, surface-active molecules of great industrial importance. The choice of the producer microbe, kind of substrate, and purification technique determine the chemistry of a biosurfactant and its production cost.
View Article and Find Full Text PDFStructural analysis and immunological activity of a novel low molecular weight neutral polysaccharide isolated from Hemerocallis citrina Borani.
Food Chem
December 2024
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address:
A novel neutral water-soluble polysaccharide (HCBP1-1) was isolated and purified from Hemerocallis citrina Borani by column chromatography. The fine structure of HCBP1-1 was determined by a series of physical and chemical means. HCBP1-1 was a homogeneous low molecular weight polysaccharide of 7.
View Article and Find Full Text PDFNon-affinity platform for processing knob-into-hole bispecific antibody.
Bioresour Bioprocess
December 2024
Shanghai AsymBio Biotechnology Co., Ltd, Building 8, No.12, Lane 855, Jinzheng Road, Jinshan Industrial Park, Shanghai, China.
Bispecific antibodies hold significant potential as next-generation biotherapeutics owing to their ability to simultaneously bind to two targets. However, the development of bispecific antibodies as biotherapeutics has been hindered by the high levels of byproducts produced, including both high molecular weight and low molecular weight variants. In addition, the inevitable expression of homodimers in host cells presents further obstacles to the commercial development of bispecific antibodies as therapeutics.
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