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Lower Diversity of Amylase-Trypsin Inhibitors and -Released Opioid-Containing Peptides in Ancestral Compared to Modern Wheat Varieties Assessed by Proteomics and Peptidomics.
J Agric Food Chem
January 2025
Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, 1433 Ås, Norway.
This study focused on identifying amylase-trypsin inhibitors (ATIs) in seven Norwegian-cultivated wheat varieties, including common wheat and ancestral species, and identifying potentially harmful opioid peptides within the digesta of these wheats. LC-MS/MS analysis of tryptic peptides from ATI fractions revealed that the common wheat variety Børsum exhibited the highest diversity of ATIs ( = 24), while they were less represented in tetraploid emmer ( = 11). Hexaploid wheat Bastian showed low diversity and relative abundance of ATIs.
View Article and Find Full Text PDFFamily Poultry Farming in Sri Lanka: An Integral Component of Protein Security, Poverty Alleviation and Public Health.
Vet Med Sci
January 2025
Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Central, Sri Lanka.
Family poultry farming plays a crucial role in ensuring protein availability and household income, particularly in low-income families. This study investigates the knowledge, attitudes and practices of family poultry farmers regarding poultry diseases, biosecurity and vaccinations. The research involved surveying 150 farmers in a selected area in Sri Lanka's western province, revealing significant knowledge gaps, particularly in understanding poultry diseases, their causes, transmission pathways and potential impacts on humans.
View Article and Find Full Text PDFNeoadjuvant chemoradiotherapy up-regulates PD-L1 in radioresistant colorectal cancer.
Clin Transl Radiat Oncol
March 2025
Institute of Medical Science & Institute for Cancer Research, Keimyung University, Daegu, Republic of Korea.
Background: Combining radiotherapy (RT) with immune checkpoint inhibitors (ICIs) is a promising strategy that can enhance the therapeutic efficacy of ICIs. However, little is known about RT-induced changes in the expression of immune checkpoints, such as PD-L1, and their clinical implications in colorectal cancer (CRC). This study aimed to investigate the association between responsiveness to RT and changes in PD-L1 expression in human CRC tissue and cell lines.
View Article and Find Full Text PDFCustomizable large-scale HPLC fraction collection using low-cost 3D printing.
HardwareX
March 2025
Molecular and Systems Pharmacology Program, Emory University, Atlanta, GA, USA.
High-performance liquid chromatography (HPLC) is an invaluable technique that has been used for many decades for the separation of various molecules. The reproducible collection of eluates from these systems has been significantly improved via its automation by fraction collection systems. Current commercially available fraction collectors are not easily customizable, incompatible with other platforms, and come with a large cost barrier making them inaccessible to many researchers.
View Article and Find Full Text PDFOptimal sandwich panel's core design for an enhanced impact resistance.
Heliyon
January 2025
Department of Industrial Engineering, College of Engineering, American University of Sharjah, P.O. Box 26666, Sharjah, United Arab Emirates.
Despite the extensive literature revealing various core structures that can enhance the impact resistance of composite panels, a comparative study illustrating the difference in performance of the various cores under same loading conditions is missing. The aim of this study is to determine the optimal core structure and design in terms of energy absorption under low-velocity impact using both numerical simulations and experimental testing for validation. Response surface analysis was used to design the experiments and analyse the panel's behaviour.
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