This study was designed to investigate by SEM the ultrastructure of the dentinal bridge formed upon pulp capping and its attachment to the contiguous dentinal walls. The pulps of 20 sound premolars and third molars were deliberately exposed and capped with a calcium hydroxide paste, Pulpdent. After 4 to 15 weeks the teeth were extracted. SEM observations suggested complete bridging, increasing in thickness after succeeding postoperative periods. Cross-sections of the specimens older than 6 weeks showed the presence of three different layers within the bridges: the superior amorphous layer was composed of tissue debris and Ca(OH)2, in the medial portion a coarse meshwork of fibers was identified as fibrodentine and the pulpal aspects exhibited tubularlike orthodentinal structures. The bridge attachment was secured by bundles of fibres linking the intertubular dentine of the pulpal walls with the dentine of the neodentinal bridge.
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Morphologie
January 2025
Department of Biostatistics, KS Hegde Medical Academy, Nitte (deemed to be university), Mangalore, Karnataka, India.
Introduction: In the forensic field, having accurate understanding of the macroscopic and microscopic alterations that occur in teeth when exposed to temperatures has remarkable significance. The preservation of delicate incinerated teeth is crucial in fire investigations that pertain to the temperature exposed, as well as the identification of victims. This preservation is necessary in order to conduct macroscopic and microscopic ultra-structural examinations, which provide valuable insights into the structural alterations that dental tissues undergo when exposed to low to high temperatures.
View Article and Find Full Text PDFPharmaceutics
December 2024
VitroScreen s.r.l., In Vitro Innovation Center, Via Mosè Bianchi 103, 20149 Milan, MI, Italy.
Skin wound healing is a physiological process orchestrated by epithelial and mesenchymal cells able to restore tissue continuity by re-organizing themselves and the ECM. This research study aimed to develop an optimized in vitro experimental model of full-thickness skin, to address molecular and morphological modifications occurring in the re-epithelization and wound healing process. Wound healing starting events were investigated within an experimental window of 8 days at the molecular level by gene expression and immunofluorescence of key epidermal and dermal biomarkers.
View Article and Find Full Text PDFInsects
November 2024
School of Agriculture, Ningxia University, Yinchuan 750021, China.
Morphological studies of the proboscis can provide valuable insights into the evolutionary adaptations of Lepidoptera. However, such research is relatively limited for Pieridae, a family that is significant both ecologically and economically. In this study, scanning electron microscopy (SEM) was employed to investigate the proboscis and associated sensilla of adult , with an emphasis on ultrastructural details and potential sex-specific differences.
View Article and Find Full Text PDFJ Clin Med
December 2024
Eye Clinic, Department of Neurosciences, Reproductive and Odontostomatological Sciences, Federico II University, 80131 Naples, Italy.
Microvilli are bristle-like protuberances of the plasma membrane, which express the vitality of mucous and epithelial cells; their alteration indicates a condition of cellular suffering in a predictive sense, making it possible to establish how much an inflammatory state or toxic conditions affect cellular functionality. In this article, the authors evaluate the applications of scanning electron microscopy (SEM) examination to impression cytology (IC) of the bulbar conjunctiva for the assessment of microvillar alteration as an early ultrastructural indicator of ocular surface health. This method offers several advantages, starting with its simplicity: it involves the non-invasive application of a strip of bibulous paper to the bulbar or tarsal conjunctiva.
View Article and Find Full Text PDFLife (Basel)
December 2024
Institute of Histology and Embryology, Faculty of Medicine, Comenius University in Bratislava, Špitálska Street 24, 813 72 Bratislava, Slovakia.
The human pineal gland is the largest producer of the hormone melatonin. Pineal acervuli (brain sand), calcified concretions in the pineal gland, have long been studied because of their association with ageing, melatonin production, and neurological disorders. The solid inorganic matter of the hydroxyapatite crystals often renders sample sectioning impossible, to the extent that the sections lose value.
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