The nucleotide sequence of the tRNALeuIAG from a lactating cow mammary gland was determined by ultramicrospectrophotometrical method and rapid gel sequencing procedure. The chain length of this tRNA is 85 nucleotides, 15 of them including 6 psi, are modified nucleotides. The primary structure of tRNALeuIAG is absolutely identical to major species of leucyl tRNAIAG from bovine liver and differs in 21 positions from cow mammary gland tRNALeuCAG.
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