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Extraction, Annotation, and Purification of Resin Glycosides from the Morning Glory Family (Convolvulaceae).
Methods Mol Biol
January 2025
Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, USA.
The metabolic diversity of plants, comprising over a million different metabolites across the plant kingdom, harbors enormous potential for pharmaceutical and biotechnological applications. Resin glycoside (RG) acylsugars from the Convolvulaceae are of interest due to their medicinal and agricultural potential. However, understanding the biological relevance of RGs is challenging as they exhibit a high lineage-specific structural diversity.
View Article and Find Full Text PDFBioremediation of alkane-containing saline soils using the long-chain alkane-degrading bacterium Pseudomonas aeruginosa DL: Effects, communities, and networks.
J Hazard Mater
January 2025
Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education) and Dalian POCT Laboratory, School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China. Electronic address:
Remediation of soil contaminated with long-chain hydrocarbons and affected by salinization poses a considerable challenge. The isolation of a bacterial strain, identified as Pseudomonas aeruginosa DL, from petroleum-contaminated saline-alkali soil has been reported in this study. The strain demonstrated a high capacity to degrade long-chain alkanes and exhibited adaptability to saline-alkali conditions.
View Article and Find Full Text PDFUsing DIMet for Differential Analysis of Labeled Metabolomics Data: A Step-by-step Guide Showcasing the Glioblastoma Metabolism.
Bio Protoc
January 2025
University of Bordeaux, CNRS, IBGC UMR 5095, Bordeaux, France.
Stable-isotope resolved metabolomics (SIRM) is a powerful approach for characterizing metabolic states in cells and organisms. By incorporating isotopes, such as C, into substrates, researchers can trace reaction rates across specific metabolic pathways. Integrating metabolomics data with gene expression profiles further enriches the analysis, as we demonstrated in our prior study on glioblastoma metabolic symbiosis.
View Article and Find Full Text PDFAortic regurgitation is the third most common valve lesion with increasing prevalence secondary to an ageing population. Transthoracic echocardiography plays a vital role in the identification and assessment of aortic regurgitation and proves essential in monitoring severity and determining the timing of intervention. Building on the foundations of previous British Society of Echocardiography (BSE) recommendations, this BSE guideline presents an update on how to approach an echocardiographic assessment of aortic regurgitation.
View Article and Find Full Text PDFAptazyme-directed A-to-I RNA editing.
Methods Enzymol
January 2025
Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Science, Chengdu, P.R. China. Electronic address:
As a promising therapeutic approach, the RNA editing process can correct pathogenic mutations and is reversible and tunable, without permanently altering the genome. RNA editing mediated by human ADAR proteins offers unique advantages, including high specificity and low immunogenicity. Compared to CRISPR-based gene editing techniques, RNA editing events are temporary, which can reduce the risk of long-term unintended side effects, making off-target edits less concerning than DNA-targeting methods.
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