The phosphorothioylating agent which was obtained by treating 2,5-dichlorophenyl phosphorodichloridothioate with 1-hydroxy-6-nitrobenzotriazole proved to be very effective for the synthesis in solution and on a solid support of phosphorothioate-containing DNA fragments.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC320440PMC
http://dx.doi.org/10.1093/nar/12.23.9095DOI Listing

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