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A Review of the Utility of Established Cell Lines for Isolation and Propagation of the Southern African Territories Serotypes of Foot-and-Mouth Disease Virus.
Viruses
December 2024
Department of Biological Sciences and Biotechnology, School of Life Sciences, Botswana International University of Science and Technology, Private Bag 16, Palapye 10071, Botswana.
Cell culture underpins virus isolation and virus neutralisation tests, which are both gold-standard diagnostic methods for foot-and-mouth disease (FMD). Cell culture is also crucial for the propagation of inactivated foot-and-mouth disease virus (FMDV) vaccines. Both primary cells and cell lines are utilised in FMDV isolation and propagation.
View Article and Find Full Text PDFPrevalence and Zoonotic Risk of Multidrug-Resistant in Bovine Subclinical Mastitis Milk: Insights Into the Virulence and Antimicrobial Resistance.
Food Sci Nutr
January 2025
Department of Dairy Science, Faculty of Veterinary, Animal and Biomedical Sciences Sylhet Agricultural University Sylhet Bangladesh.
The emergence of antibiotic-resistant microorganisms has made antimicrobial resistance a global issue, and milk is a potential source for the propagation of resistant bacteria causing zoonotic diseases. Subclinical mastitis (SCM) cases, often overlooked and mixed with normal milk in dairy farms, frequently involve , which can spread through contaminated milk. We conducted this study to determine the prevalence of virulence genes, antibiotic resistance genes (ARGs), antimicrobial susceptibility, and the genetic relatedness of multidrug-resistant (MDR) Shiga toxin-producing (STEC) isolated from SCM milk.
View Article and Find Full Text PDFThe Safety and Efficacy of New DIVA Inactivated Vaccines Against Lumpy Skin Disease in Calves.
Vaccines (Basel)
November 2024
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale", 64100 Teramo, Italy.
Lumpy skin disease virus ( family- genus) is the aetiological agent of LSD, a disease primarily transmitted by hematophagous biting, affecting principally cattle. Currently, only live attenuated vaccines are commercially available, but their use is limited to endemic areas. There is a need for safer vaccines, especially in LSD-free countries.
View Article and Find Full Text PDFDevelopment and Characterisation of an Immortal Cell Line From Largemouth Bass (Micropterus salmoides) for Viral Studies.
J Fish Dis
December 2024
Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture, Pearl River Fishery Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
Article Synopsis
- Researchers successfully created an immortal cell line (MSF) from the fin tissue of Largemouth bass, which can be cultured continuously for over 80 passages.
- The MSF cells, maintained at 28°C and confirmed to be free from mycoplasma contamination, are identified as epithelialoid cells with specific chromosome characteristics.
- This new cell line is highly susceptible to various viruses affecting Largemouth bass, making it a valuable tool for future studies on gene expression and viral mechanics.
infection specifically suppresses the expression of a host lncRNA to facilitate parasite propagation by modulating host cell mitochondrial function and autophagy.
Microbiol Spectr
December 2024
Department of Parasitology, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
is one of the most common pathogens causing reproductive failure in ruminants (e.g., cattle and goats) worldwide.
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