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A sensitive post-column derivatization approach for enhancing hydroxyl metabolites detection.
Anal Chim Acta
February 2025
Department of Pharmacy, College of Pharmaceutical Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan. Electronic address:
Background: Chemical derivatization is a common technique in liquid chromatography-mass spectrometry (LC-MS) metabolomics used to improve the ionizability and chromatographic properties of metabolites in complex biological samples. This process facilitates better detection and separation of a wide array of compounds. The reagent 2-(4-boronobenzyl) isoquinolin-2-ium bromide (BBII), developed as a glucose labeling reagent for matrix-assisted laser desorption/ionization MS, enhances ionization for glucose and other hydroxyl metabolites.
View Article and Find Full Text PDFDevelopment of a Specific Fluorescence Post-column Derivatization Method Coupled with Ion-Pair Chromatography for Phytate Analysis in Food.
J Agric Food Chem
January 2025
Qualisud, Université Montpellier, CIRAD, Montpellier SupAgro, Université d'Avignon, Université de La Réunion, Montpellier 34093, France.
Phytate in plants (inositol phosphates, InsPs) affects mineral bioavailability. However, methods for their quantification may lead to variable results, and some are nonspecific (spectrophotometric techniques). In this study, ion-pair high-performance liquid chromatography (HPLC) was coupled with post-column derivatization to allow fluorescence detection (FLD, λ324/λ364 nm) of InsPs.
View Article and Find Full Text PDFDetermination of adalimumab by HPLC with fluorescence detection using the König reaction.
Talanta
January 2025
Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo, Chiba, Chiba, 60-8675, Japan. Electronic address:
Monoclonal antibody drugs (mAb) are widely used to treat various diseases. Keeping quality of mAbs is crucial to maximize efficacy and minimize adverse effects. To this end, mAb content in the drug product must be precisely quantified.
View Article and Find Full Text PDFDopamine-supported HPLC post-column derivatization to fluorescence: Simultaneous and sensitive detection of eight tea polyphenols.
Food Chem
February 2025
College of Chemistry, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address:
The effective differentiation and detection of multiple tea polyphenols are often challenging due to their subtle structural similarities. Although post-column derivatization HPLC strategies are commonly employed to distinguish multiple targets, the short physical distance between chromatographic column and detector limits reaction time, thereby reducing the derivatization efficiency. Dopamine (DA) reacts rapidly with resorcinol to form fluorescent azamonardine products, making fast fluorometric derivatization of tea polyphenols containing resorcinol motifs possible.
View Article and Find Full Text PDFTetrodotoxin Derivatization with a Newly Designed Boron Reagent Leads to Conventional Reversed-Phase Liquid Chromatography.
Toxins (Basel)
June 2024
National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki 210-9501, Japan.
Article Synopsis
- Tetrodotoxin (TTX) is a harmful toxin associated with pufferfish poisoning, predominantly in East and Southeast Asia, and traditional detection methods have limitations.
- Researchers developed a new analytical method for TTX using pre-column derivatization with a compound called NBD-H-DAB and reversed-phase high-performance liquid chromatography (RP HPLC) for quicker assessments.
- The new method showed effective sensitivity, achieving a detection limit of 0.06 mg/kg in fish samples, demonstrating the potential for improved TTX analysis.
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