Observations of the reactivation rate and subsequent growth in addition to the luminescence of cells subjected to supercooling and freezing at 15 degrees C suggested that the effect of supercooling is more significant than that of freezing. In supercooled suspensions, cells are more injured than in frozen ones and the number of injured cells increases more rapidly. Unlike the supercooled cells, the live cells from frozen suspensions maintain a higher growth activity for a longer time.
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