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Separation of deaminated impurities from the desired oligonucleotides using supercritical fluid chromatography.
J Chromatogr A
January 2025
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address:
With recent advancements concerning the optimization of the analytical conditions, it is feasible to analyze polar molecules using supercritical fluid chromatography (SFC). In this study, the applicability of SFC is evaluated for analyzing 5-, 10-, 15-, and 18-mer oligonucleotides, and SFC is then applied to analyze deaminated products, which are side products generated during oligonucleotide synthesis. These side products are difficult to separate from the target oligonucleotide, with the difficulty varying depending on the deamination position and sequences, even when using ion-pair reversed-phase liquid chromatography (IP-RPLC), a common method for oligonucleotide analysis.
View Article and Find Full Text PDFIsolation and Identification of Coumarins from Citrus latifolia Leaves Using Advanced Chromatographic Techniques.
Methods Mol Biol
January 2025
Laborotorio de Química de Products Naturales, Departmento de Biotecnologia y Bioquímica, Centro de Investigatión y de Estudios Avanzados de IPN, Guanajuato, Mexico.
Coumarins are a class of natural compounds that exhibit a wide range of biological activities, which include anticoagulant, anti-inflammatory, anticancer, and antimicrobial effects. This class of compounds has drawn considerable interest in recent years. The exploration of such properties contributes to our understanding of the potential of coumarins as valuable agents in various clinical applications.
View Article and Find Full Text PDFHigh-Resolution Isolation of Natural Products: Efficient Combination of Dryload Injection and HPLC Gradient Transfer.
Methods Mol Biol
January 2025
School of Pharmaceutical Sciences, University of Geneva, Geneva, Switzerland.
The isolation of pure compounds from complex extracts is a crucial step in natural products (NPs) research. Historically, this process has been recognized to be slow and laborious. However, significant advancements have been made in isolation methods.
View Article and Find Full Text PDFNatural Products Analysis Through Time: From Past Achievements to Future Prospects.
Methods Mol Biol
January 2025
Natural Products Lab, Institute of Biology, Leiden University, Leiden, The Netherlands.
This introductory chapter traces the evolution of (bio)chemical assays, emphasizing the critical role of robust protocols in ensuring reproducibility-a fundamental aspect of scientific research. With the advent of systems biology, the need for standardized methods has intensified, particularly for integrating vast datasets in open-access formats. The historical progression from basic plant morphology to advanced chromatographic and spectroscopic techniques in phytochemistry highlights the necessity for precise, reproducible protocols.
View Article and Find Full Text PDFThe metabolome of Sphingobium chinhatense IP26 exposed to chlorinated paraffins - Efficient data analysis with RASER.
Chemosphere
January 2025
Empa, Swiss Federal Laboratories for Materials Testing and Research, Laboratory for Advanced Analytical Technologies, Überlandstrasse 129, CH-8600, Dübendorf, Switzerland. Electronic address:
The universe of possible chloro-paraffin (CP) structures is a complex one. Even the world of short-chain CPs (SCCPs) is large, containing thousands of constitutional isomers and stereoisomers. We investigated a technical SCCP mixture (Hordalub 80, Vantage Leuna, m = 56%) and found 33 CP-homologues in this material with carbon- (n) and chlorine-numbers (n) varying from 10 to 13 and 4-12, respectively.
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