An increased level of glycoproteins (seromucoid, protein-bound hexoses, protein-bound hexosamines, protein-bound sialic acids) was found in the blood serum from patients with rheumatoid arthritis (RA) as compared with healthy individuals. Higher glycoprotein content was observed in the seropositive RA patients than in the seronegative ones. Higher levels of glycoproteins was also observed in the synovial fluid from seropositive RA patients than in the seronegative ones.
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Arthroplast Today
December 2024
Hospital Pavia Arecibo, Arecibo Orthopedic Institute, Arecibo, PR, USA.
Synovial fistula of the knee occurs when a defect in the joint capsule creates a connection between skin, bursa, and a near tissue, manifesting as an opening with continuous draining fluid or a cyst. This is a case of an 80-year-old male who developed a synovial fistula with cystic presentation 6 years after the primary total knee arthroplasty. Management included a total knee arthroplasty revision surgery with intraoperative identification of the fistula with methylene blue, and using a gelatin-thrombin matrix for closure.
View Article and Find Full Text PDFACS Appl Bio Mater
January 2025
Department of Materials Engineering, Indian Institute of Science Bangalore, Karnataka 560012, India.
The cartilage possesses limited regenerative capacity, necessitating advanced approaches for its repair. This study introduces a bioink designed for cartilage tissue engineering (TE) by incorporating ionically cross-linkable alginate into the photo-cross-linkable MuMA bioink, resulting in a double cross-linked interpenetrating network (IPN) hydrogel. Additionally, hyaluronic acid (HA), a natural component of cartilage and synovial fluid, was added to enhance the scaffold's properties.
View Article and Find Full Text PDFAnn Transl Med
December 2024
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA.
Background: Osteoarthritis (OA) is increasingly thought to be a multifactorial disease in which sustained gut inflammation serves as a continued source of inflammatory mediators driving degenerative processes at distant sites such as joints. The objective of this study was to use the equine model of naturally occurring obesity associated OA to compare the fecal microbiome in OA and health and correlate those findings to differential gene expression synovial fluid (SF) cells, circulating leukocytes and cytokine levels (plasma, SF) towards improved understanding of the interplay between microbiome and immune transcriptome in OA pathophysiology.
Methods: Feces, peripheral blood mononuclear cells (PBMCs), and SF cells were isolated from healthy skeletally mature horses (n=12; 6 males, 6 females) and those with OA (n=6, 2 females, 4 males).
Commun Biol
January 2025
Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China.
Protein citrullination modification plays a pivotal role in the pathogenesis of rheumatoid arthritis (RA), and anti-citrullinated protein antibodies (ACPAs) are extensively employed for clinical diagnosis of RA. However, there remains limited understanding regarding specific citrullinated proteins and their implications in the progression of RA. In this study, we screen and verify insulin-like growth factor-2 mRNA binding protein 1 (IGF2BP1) as a novel citrullinated protein with significantly elevated citrullinated level in RA.
View Article and Find Full Text PDFIDCases
December 2024
Maimonides Medical Center, Brooklyn, NY, USA.
An 85-year-old woman with a history of total knee replacements for osteoarthritis in the past, presented with left knee swelling and pain that persisted for 14 months. An initial diagnosis of synovial cyst was made, and she underwent multiple aspirations and symptomatic treatments without improvement. Repeat arthrocentesis showed a WBC of 56,000/μL with 61 % neutrophils and 34 % lymphocytes.
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