We have developed a simple new method of rotary shadowing, double-axis rotary replication, for observing three-dimensional structures in deep-etched, rapid-frozen tissues. The technical details of this method are described and compared with the conventional fixed-angle rotary shadowing procedure.
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http://dx.doi.org/10.1111/j.1365-2818.1984.tb02552.x | DOI Listing |
J Electron Microsc Tech
August 1991
Laboratory for Electron Microscopy I, Institute for Cell Biology, ETH Zentrum, Zürich, Switzerland.
Structural information on the surface of biological specimens can be resolved within molecular dimensions by "in-lens" field emission scanning electron microscopes when cryo-methods are used to adequately preserve the native state of the specimen. The visual definition of molecular surface structures depends largely on the metal coating. The thickness of the coating, as well as the temperature at which it is deposited, are among the most important parameters affecting visual definition.
View Article and Find Full Text PDFAm J Physiol
September 1985
We have compared the ultrastructures of the cytoplasmic surfaces (CS) of isolated, glutaraldehyde-fixed gap junctional pellets from rat ventricles and liver by rapid freezing on a liquid helium-cooled surface, freeze fracture, deep etching, and double-axis rotary replication (J. Microsc. Oxford 137: 121-123, 1984).
View Article and Find Full Text PDFWe have developed a simple new method of rotary shadowing, double-axis rotary replication, for observing three-dimensional structures in deep-etched, rapid-frozen tissues. The technical details of this method are described and compared with the conventional fixed-angle rotary shadowing procedure.
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