We have developed a simple new method of rotary shadowing, double-axis rotary replication, for observing three-dimensional structures in deep-etched, rapid-frozen tissues. The technical details of this method are described and compared with the conventional fixed-angle rotary shadowing procedure.

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http://dx.doi.org/10.1111/j.1365-2818.1984.tb02552.xDOI Listing

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View Article and Find Full Text PDF

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