AI Article Synopsis
- The study analyzed how T7 phage DNA interacts with different phospholipids using 31P NMR, focusing on a specific PL to nucleotide ratio.
- The researchers estimated the contributions of both phospholipids and DNA to the NMR spectrum, highlighting that the interaction led to partial immobilization of the phospholipids.
- The strength of these interactions varied by phospholipid type, showing a trend where phosphatidylcholine and phosphatidylethanolamine were less effective than sphingomyelin and a mixture of the three phospholipids.
Article Abstract
The phage T7 DNA complexes with various phospholipids (PL) were studied by 31P NMR at PL/nucleotide molar ratio of 2 : 1. Using a phosphatidylcholine thion analogue, the contributions of PL and DNA into the 31P NMR spectrum of the complex were estimated. It was found that PL-DNA interaction results in partial immobilizing ability of PL depends on their structure, increasing in the following row: phosphatidylcholine-phosphatidylethanolamine less than sphingomyelin less than or equal to ternary mixture of these PL. The data obtained are indicative of nonequivalent binding of DNA with various PL species.
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