Glycolipid synthetases can be assayed conveniently by incubating the lipid substrate with the radiosugar-labeled nucleotide in a small plastic scintillation vial. At the end of the incubation period, water and perchloric acid are added, then n-butanol, then a toluene-based scintillation cocktail. The radioactive lipid partitions into the scintillation fluid, leaving excess sugar nucleotide in the aqueous phase. Only a small fraction of the total radioactivity in the aqueous layer is detectable. This method is illustrated for ceramide:UDP-glucose glucosyltransferase. The approach should be applicable to other lipid synthetases that can be assayed with radioactive hydrophilic substrate.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/BF02535347 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Integrated genome and metabolome mining unveiled structure and biosynthesis of novel lipopeptides from a deep-sea Rhodococcus.
Microb Biotechnol
November 2024
Department of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Giardini del Molosiglio, Naples, Italy.
Microbial biosurfactants have garnered significant interest from industry due to their lower toxicity, biodegradability, activity at lower concentrations and higher resistance compared to synthetic surfactants. The deep-sea Rhodococcus sp. I2R has been identified as a producer of glycolipid biosurfactants, specifically succinoyl trehalolipids, which exhibit antiviral activity.
View Article and Find Full Text PDFAcinetobacter corruptisaponis sp. nov., Isolated from a Spoiled Bath Lotion.
Curr Microbiol
October 2024
Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, People's Republic of China.
Strain DM2021935 representing a novel Acinetobacter species was isolated from a spoiled bath lotion in Guangdong, China. Based on 16S rRNA gene phylogenetic analysis, strain DM2021935 was closely related to 'Acinetobacter thutiue' VNH17, Acinetobacter junii CIP 64.5, and Acinetobacter tibetensis Y-23.
View Article and Find Full Text PDFshRNA-mediated down-regulation of Acsl1 reverses skeletal muscle insulin resistance in obese C57BL6/J mice.
PLoS One
August 2024
Department of Medical Biology, Medical University of Bialystok, Bialystok, Poland.
Prolonged consumption of diet rich in fats is regarded as the major factor leading to the insulin resistance (IR) and type 2 diabetes (T2D). Emerging evidence link excessive accumulation of bioactive lipids such as diacylglycerol (DAG) and ceramide (Cer), with impairment of insulin signaling in skeletal muscle. Until recently, little has been known about the involvement of long-chain acyl-CoAs synthetases in the above mechanism.
View Article and Find Full Text PDFCadmium exacerbates liver injury by remodeling ceramide metabolism: Multiomics and laboratory evidence.
Sci Total Environ
May 2024
The First Clinical Medical College of Lanzhou University, Lanzhou 730000, China; Department of General Surgery, the First Hospital of Lanzhou University, Lanzhou 730000, China; Key Laboratory of Biotherapy and Regenerative Medicine of Gansu Province, Lanzhou 730000, China; Center for Cancer Prevention and Treatment, School of Medicine, Lanzhou University, Lanzhou 730000, China; Gansu Provincial Institute of Hepatobiliary and Pancreatic Surgery, Lanzhou 730000, China. Electronic address:
Cadmium (Cd) is a toxic heavy metal that primarily targets the liver. Cd exposure disrupts specific lipid metabolic pathways; however, the underlying mechanisms remain unclear. This study aimed to investigate the lipidomic characteristics of rat livers after Cd exposure as well as the potential mechanisms of Cd-induced liver injury.
View Article and Find Full Text PDFTherapeutic Strategy for Fabry Disease by Intravenous Administration of Adeno-Associated Virus 9 in a Symptomatic Mouse Model.
Hum Gene Ther
March 2024
Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Japan.
Fabry disease (FD) is an inherited lysosomal storage disease caused by deficiency of α-galactosidase A (α-Gal A), an enzyme that hydrolyzes glycosphingolipids in lysosome. Accumulation of glycosphingolipids, mainly globotriaosylceramide (Gb3) in tissues, induces cellular dysfunction leading to multi-organ disorder. Gene therapy is a promising strategy that can overcome these problems, and virus vectors such as adeno-associated virus (AAV) have been used for study on gene therapy.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!