A method for the ethanol fractionation of normal mouse serum has been developed which is rapid and simple to perform. The method was shown to be effective in purification of IgG1, IgG2 and gammaM-gammaA globulins. Gel filtration and zone electrophoresis proved to be effective in separating the gammaM-gammaA globulin combination into their respective components. Immunoelectrophoresis was chosen to assess the homogeneity of the fractions and the results agree with those obtained by other workers.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0022-1759(76)90076-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Ultrasound pretreatment and solvent extraction parameters effects on the nutritional characteristics of Indonesian shortfin eel () protein concentrate.
Food Chem X
January 2025
Department of Food Technology, Universiti Putra Malaysia, Serdang 43400, Malaysia.
Protein concentrate (PC) is a potential solution to address the global protein shortage, with Indonesian shortfin eel being a suitable raw material. This research investigates the impact of ultrasound pretreatment and extraction parameters on the nutritional quality of eel protein concentrate (EPC). The study involved ultrasonic pretreatment at different times and power, and solvent extraction with different solvents, temperature, and solvent-solid-feed-ratio (SSFR).
View Article and Find Full Text PDFEvaluation of the antiulcer activity of ethanolic extract and solvent fractions of Rupr. in experimental rats and their DART-ToF-MS profiling.
Nat Prod Res
January 2025
Advanced Materials Research Chair, Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
In this study, antiulcer activity of ethanolic extract and solvent fractions of the aerial part of was investigated using ethanol-induced model of gastric ulceration in rats. The results showed that ethyl acetate, non-polar components and diethyl ether fractions have a remarkable antiulcerogenic activity; because they exhibited control-ulcer protection by 85.2%, 77.
View Article and Find Full Text PDFBranched-Chain Amino Acid (BCAA) Oligopeptide Determination from Whey Proteins: Preparation, Peptide Profiles, and Anti-Fatigue Activity.
Foods
December 2024
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Whey proteins have anti-fatigue activity, but there are few studies that have reported the ameliorative effects of branched-chain amino acid (BCAA) oligopeptides from whey proteins on fatigue in mice. The purposes of this study were to establish a process for the preparation of BCAA oligopeptides from whey protein and to investigate the anti-fatigue activity of BCAA oligopeptides. Whey proteins were hydrolyzed by trypsin and flavourzyme and purified by ethanol precipitation and reversed-phase high performance liquid chromatography (RP-HPLC).
View Article and Find Full Text PDFOptimization of a Pressurized Extraction Process Based on a Ternary Solvent System for the Recovery of Neuroprotective Compounds from Leaves.
Int J Mol Sci
December 2024
Laboratory of Organic Chemistry LR17ES08, Faculty of Sciences of Sfax, University of Sfax, B.P 1171, Sfax 3038, Tunisia.
Green chemistry focuses on reducing the environmental impacts of chemicals through sustainable practices. Traditional methods for extracting bioactive compounds from leaves, such as hydro-distillation and organic solvent extraction, have limitations, including long extraction times, high energy consumption, and potential toxic solvent residues. This study explored the use of supercritical fluid extraction (SFE), pressurized liquid extraction (PLE), and gas-expanded liquid (GXL) processes to improve efficiency and selectivity.
View Article and Find Full Text PDFMicrobial production of food lipids using the oleaginous yeast Apiotrichum brassicae.
Food Res Int
January 2025
VTT Technical Research Centre of Finland, Tekniikantie 21, 02044 VTT Espoo, Finland. Electronic address:
Oleaginous yeasts offer a promising sustainable alternative for producing edible lipids, potentially replacing animal and unsustainable plant fats and oils. In this study, we screened 11 oleaginous yeast species for their lipid profiles and identified Apiotrichum brassicae as the most promising candidate due to its versatility across different growth media. A.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!