The process of collagen and proteoglycan synthesis in the in vitro cultured fibroblasts was studied by four original histoenzymological methods: UDP-glucuronic-5-epimerase, hydroxyproline epimerase, UDP-4-epimerase and UDP-N-acetyl-glucosamine epimerase. The process of epimerasization varied in terms of the cell growth stages. Synthesis of collagen and proteoglycans developed in the cells of the same culture but with various intensities in different cell groups.
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J Vis Exp
January 2025
Department of Biology, Mount Saint Vincent University;
Zebrafish scales offer a variety of advantages for use in standard laboratories for teaching and research purposes. Scales are easily collected without the need for euthanasia, regenerate within a couple of weeks, and are translucent and small, allowing them to be viewed using a standard microscope. Zebrafish scales are especially useful in educational environments, as they provide a unique opportunity for students to engage in hands-on learning experiences, particularly in understanding cellular dynamics and in vitro culturing methods.
View Article and Find Full Text PDFBraz J Microbiol
January 2025
Department of Botany, Gauhati University, Gopinath Bordoloi Nagar, Jalukbari, Guwahati, 781014, Assam, India.
Plant-associated microbiome plays important role in maintaining overall health of the host plant. Xanthium strumarium displaying resilience to various environmental fluctuations may harbor some bacterial isolates which can help this plant to grow worldwide. The present study aims to isolate endophytic and rhizospheric bacteria from X.
View Article and Find Full Text PDFBraz J Microbiol
January 2025
Programa de Pós-Graduação em Produção Vegetal no Semiárido, Universidade Estadual de Montes Claros, Rua Reinaldo Viana, 2650, Janaúba, MG, 39400-000, Brazil.
The objective of this work was to investigate the biofilm production capacity of the isolate EB-40 (Bacillus cereus) in a culture medium for the multiplication of microorganisms and in roots of in vitro grown banana explants. It was observed that the isolate was able to produce biofilms in tryptone, soy and agar (TSA) culture medium and in the roots of explants. The format, architecture and location of the biofilms in TSA culture medium presented an exopolymer matrix formed by EB-40 presented coccoid bacillary cells and fibrillar structures.
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January 2025
Department of Urology, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Renal fibrosis is a common pathological process in various chronic kidney diseases. The accumulation of senescent renal tubular epithelial cells (TECs) in renal tissues plays an important role in the development of renal fibrosis. Eliminating senescent TECs has been proven to effectively reduce renal fibrosis.
View Article and Find Full Text PDFDis Model Mech
January 2025
Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Science, Radboud University, Nijmegen 6525GA, The Netherlands.
Hepatic organoid cultures are a powerful model to study liver development and diseases in vitro. However, hepatocyte-like cells differentiated from these organoids remain immature compared to primary human hepatocytes (PHHs), which are the benchmark in the field. Here, we applied integrative single-cell transcriptome and chromatin accessibility analysis to reveal gene regulatory mechanisms underlying these differences.
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